Monoclonal antibody for II type carp herpes virus ORF72 albumen and application thereof

A technology of monoclonal antibody and carp herpes virus, which is applied in the direction of antiviral immunoglobulin, virus antigen components, virus/bacteriophage, etc., can solve problems that have not been reported, and achieve high sensitivity and strong specificity

Inactive Publication Date: 2017-02-01
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But about the monoclonal antibody of a strain II type cyprin herpesvirus ORF72 protein of the present invention and its application, there is no report yet

Method used

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  • Monoclonal antibody for II type carp herpes virus ORF72 albumen and application thereof
  • Monoclonal antibody for II type carp herpes virus ORF72 albumen and application thereof
  • Monoclonal antibody for II type carp herpes virus ORF72 albumen and application thereof

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Experimental program
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Effect test

Embodiment 1

[0022] The preparation of embodiment 1 polyclonal antibody

[0023] 1.1 Experimental instruments and materials

[0024] The recombinant plasmid PGEX-4T-3-ORF72 containing the ORF72 gene of the CyHV-2-YC strain was constructed and preserved by our laboratory; Escherichia coli DH5α and BL21 (DE3) were purchased from Tiangen Biological Co., Ltd.; restriction enzymes , PCR kit, and T4 ligase were purchased from Takara Company; HRP-labeled goat anti-mouse IgG and goat anti-rabbit IgG polyclonal antibodies were purchased from Beyond Biotech Co., Ltd.; 6-week-old ICR mice were purchased from Shanghai Experimental Animal Research Center; 2-8°C refrigerator: Haier; -20°C low-temperature freezer: Haier; -80°C low-temperature freezer: Zhongke Meiling; -150°C ultra-low temperature freezer: Thermo; bacterial electric heating constant temperature incubator: Shanghai Anting Technology Instrument Co., Ltd. Company; low temperature and constant temperature bath: Shanghai Hede Test Equipment C...

Embodiment 2

[0037] The preparation of embodiment 2 monoclonal antibody

[0038] 2.1 SP2 / 0 mouse myeloma cells and experimental mice

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Abstract

The invention relates to a monoclonal antibody for II type carp herpes virus ORF72 albumen. The monoclonal antibody is secreted by hybridoma of which the preservation number is CCTCC NO: C2016141. The hybridoma is prepared by the steps of immunizing mice with GST-ORF72 recombinant protein as antigen to obtain mice splenocyte, and fusing mice splenocyte with mice myeloma cell to obtain the hybridoma. The invention further provides the application of the monoclonal antibody. The monoclonal antibody for II type carp herpes virus ORF72 albumen has the advantages that the monoclonal antibody for II type carp herpes virus ORF72 albumen is made for the first time and can be used for preparing diagnostic reagent for II type carp herpes virus and anti-II type carp herpes virus medicine.

Description

technical field [0001] The invention relates to the technical field, in particular to a monoclonal antibody of type II carp herpesvirus ORF72 protein and its application. Background technique [0002] Crucian carp (Crucian carp) has low feeding costs and a wide range of breeding areas. It plays an important role in freshwater fish farming in my country. Crucian carp farming in my country is mainly concentrated in the eastern, central and southern regions, such as Jiangsu, Hubei, Jiangxi, Anhui, Shandong, Guangdong, Sichuan , Liaoning, Hunan, Zhejiang and other provinces. According to statistics, the annual output of crucian carp in my country reached 2,594,400 tons in 2013, and the main cultured species is Carassius auratus gibelio. In recent years, the frequent occurrence of herpesviral haematopoietic necrosis (HVHN) has seriously affected the aquaculture industry of crucian carp in my country. HVHN is a highly pathogenic viral disease of crucian carp, and its pathogen is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C12N5/20G01N33/577G01N33/569A61K39/42A61P31/22C12R1/91
CPCC07K16/085A61K39/12C12N2710/16034G01N33/56994G01N33/577
Inventor 吕利群孔善云姜有声许丹
Owner SHANGHAI OCEAN UNIV
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