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An endogenous biocontrol strain for inhibiting Acanthopanax blight and its application

A technology of Acanthopanax senticosus and blight, applied in the field of microorganisms, can solve problems such as serious harm, destruction of soil biological structure, increase in pesticide residues, etc., and achieve ecological balance protection, environmental pollution reduction, income increase and production improvement and promotion. Effect

Inactive Publication Date: 2019-02-12
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a soil-borne disease caused by fungi in the seedling stage of plants. It has a wide range of occurrence, serious damage, and difficult control. Many years of chemical control have caused pathogenic bacteria to develop resistance to various fungicides, and the development of resistance is amazing, resulting in the use of chemical pesticides. The dosage is continuously increased, resulting in the increase of pesticide residues and the destruction of soil biological structure.

Method used

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  • An endogenous biocontrol strain for inhibiting Acanthopanax blight and its application
  • An endogenous biocontrol strain for inhibiting Acanthopanax blight and its application
  • An endogenous biocontrol strain for inhibiting Acanthopanax blight and its application

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specific Embodiment approach 1

[0024] Embodiment 1: The endogenous biocontrol strain Bacillus sp. SC-JW-14 provided in this embodiment to inhibit Acanthopanax blight is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and the preservation address is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The preservation date is September 1, 2016, and the preservation number is CGMCCNo.12921.

[0025] The Bacillus sp. SC-JW-14 of this embodiment is obtained from the Acanthopanax experimental field of the Mudanjiang Forest By-products Research Institute, and is obtained by isolating endophytic bacteria from tissue materials of healthy plants. The specific method is as follows:

[0026] 1. Isolation of endophytic bacteria

[0027] After the harvested plants were rinsed with water, appropriate amount of root, stem, and leaf tissues were selected, weighed, cut into 5mm×5mm tissue pieces, soaked in 95% ethanol for 1min, and then soaked in 1% NaClO so...

specific Embodiment approach 2

[0051] Specific embodiment two: in this embodiment, bacillus (Bacillus sp.) SC-JW-14 can be used for the biological control of Acanthopanax blight, and the control effect experiment is as follows:

[0052] Three experimental groups were set up for pot experiments, which were the healthy control group, the susceptible control group and the control group, with 20 Acanthopanax senticosus plants in each group. Use Rhizoctonia solani (Acanthopanax senticosus) to prepare a concentration of 1 × 10 5 per mL of spore suspension. After surface disinfection of Acanthopanax senticosus seedlings, the healthy control group was injected with 20 μL of sterile water by acupuncture method, and the susceptible control group and control group were each injected with 20 μL of Rhizoctonia solani spore suspension and inoculated in flower pots. And the roots of the plants in the control group were irrigated with 3mL Acanthopanax biocontrol bacteria culture solution (concentration was 0.6×10 7 ~1×10...

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Abstract

The invention discloses an endogenous biocontrol strain for inhibiting Acanthopanax blight and its application. The endogenous biocontrol strain is Bacillus sp. SC‑JW‑14, which is preserved in China Microorganisms Preservation Management The General Microbiology Center of the Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.12921. The Bacillus sp. SC-JW-14 of the present invention can be used for the biological control of Acanthopanax blight, the antibacterial rate against Rhizoctonia solani can reach 69.6%, and the control effect can reach 59.4%. The present invention takes the pathogenic bacteria of Acanthopanax blight as the prevention and control object, isolates and screens the antagonistic endophytic strains existing in healthy plants Acanthopanax senticosus, lays the foundation for the biological control of Acanthopanax blight, and its rational application can reduce the number of chemical agents The environmental pollution caused is conducive to the protection of ecological balance, and can greatly improve and promote the artificial cultivation of Acanthopanax senticosus and the increase in income and production, which has certain research significance.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a bacterial strain for inhibiting Acanthopanax blight and application thereof. Background technique [0002] Acanthopanax.sentieosus Harm., a plant of the Araliaceae family, is mainly produced in Northeast my country. It is the most important medicinal material in the genus Aralis. Good health care effect. At present, Acanthopanax not only sells well in China, but also exported to Japan, South Korea, Europe, North America and other countries. It is one of the main Chinese herbal medicines that my country exports to earn foreign exchange, with an annual demand of about 5,000 tons. [0003] Due to the increase in market demand and rising prices, drug farmers have destructively excavated a large number of Acanthopanax rhizomes, and the destructive excavation has prevented them from renewing and forming communities for a long period of time, resulting in a large-scale and rapid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/04A01P3/00C12R1/07
CPCA01N63/30C12N1/205C12R2001/07
Inventor 姜威张淑梅李晶孟利强胡基华刘宇帅陈靖宇
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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