Rectal cancer radiotherapy resistance cell model and construction method therefor

A cell and cell line technology, applied in the field of cells, can solve the problems of increased ratio, inability to represent clinical recurrent cancer radioresistance, and time-consuming problems

Inactive Publication Date: 2017-02-01
李懿
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Among them, the "low-dose long-range induction method" usually repeatedly irradiates tumor cells with a dose of 2Gy for about 30 times to induce radiotherapy-resistant cell subsets in tumor cells. This process is time-consuming and requires repeated treatment of cells, increasing the The probability of contamination is reduced; and the "high-dose short-range screening method" is to increase the ratio of radioresistant cell subpopulations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] Establishment of Radiation Resistant Cell Line SW480-R

[0021] 1. Method

[0022] 1. Cell culture

[0023] SW480 cells were inoculated into culture flasks with RPMI1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Penicillin-Streptomycin) solution, and cultured in a constant temperature incubator at 37°C, CO 2 Concentration 5%, saturated humidity. When the cells grow to 80-90%, they are digested with trypsin (0.125%), counted and diluted to a certain concentration for passage.

[0024] 2. Irradiation method

[0025] Using Siemens PRIMUS linear accelerator, 6MV X-ray irradiation, the source-to-skin distance is 100cm, the absorbed dose rate is 200cGy / min, and the culture plate is covered with 1.5cm thick silica gel compensator.

[0026] 3. Establishment of radioresistant cell line SW480-R

[0027] The colon cancer SW480 cell line in the exponential growth phase was taken, and after being irradiated with 2Gy X-rays for the first time...

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Abstract

A construction method of a cell model is disclosed, by which a rectal cancer radiotherapy resistant cell strain is obtained in a manner of gradiently increasing radiation dose of X-ray, wherein the cell strain is sourced from an SW480 cell and the resistance of the resistant cell under high radiation dose and low radiation dose are both higher than those of the parent SW480 cell strain.

Description

technical field [0001] The invention belongs to the field of cells, in particular to the construction of cell models. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the incidence of rectal cancer has increased year by year. Due to the special anatomical location of the rectum, early tumors are not easy to be found, and most patients are already in the middle and advanced stages when they seek treatment. These patients have a high recurrence rate after simple surgical resection, and radiotherapy is an important local treatment. The combination with surgery can significantly reduce the local recurrence rate and improve the long-term survival of patients. However, in the course of clinical treatment, we also observed that not all rectal cancer patients are sensitive to radiotherapy. The heterogeneity of rectal cancer cells’ response to X-rays affects the clinical application and curative effect of radiotherapy. The...

Claims

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Application Information

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IPC IPC(8): C12N5/09
CPCC12N5/0693
Inventor 李懿陈宏杨祚璋李秋恬李英华黄美金宋锐苏斌
Owner 李懿
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