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Dextran sulfate sodium-induced chicken enteritis model and building method thereof

A dextran sodium sulfate and a technology for establishing a method are applied to the chicken enteritis model induced by dextran sodium sulfate and the field of its establishment, which can solve the problems of narrow effective dose range, limited application, short onset time and the like, and achieve the degree of pathological changes. Adjustable, large effective dose range, reducing economic losses

Inactive Publication Date: 2017-02-15
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above studies have a series of problems such as short onset time, too severe disease and narrow effective dose range. Oral feeding also has disadvantages such as cumbersome operation, poor repeatability and low stability, which limit its application.

Method used

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  • Dextran sulfate sodium-induced chicken enteritis model and building method thereof
  • Dextran sulfate sodium-induced chicken enteritis model and building method thereof
  • Dextran sulfate sodium-induced chicken enteritis model and building method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The establishment of the rooster enteritis model induced by embodiment 1 dextran sodium sulfate

[0027] The establishment method of the cock enteritis model induced by dextran sodium sulfate of the present embodiment is operated according to the following steps:

[0028] 1. Dissolve sodium dextran sulfate (US MPBIO product) with a molecular weight of 36-50kDa in ordinary drinking water, and prepare sodium dextran sulfate with concentrations of 0.75%, 1.25%, 1.5% and 2.5% by weight respectively solution, ready to use.

[0029] 2. Select 240 16-day-old healthy and fast-growing cocks, randomly divide them into 5 groups, each group has 3 repetitions, and each repetition has 16, and they are raised in the breeder brooding house. Raised in cages, kept under continuous light, and drank freely from nipple drinking fountains. Free feeding, the experimental basal diet is based on the national industry standard (NY / T 33-2004) according to the nutritional needs of yellow-feather...

Embodiment 2

[0043] The establishment of the hen enteritis model induced by embodiment 2 dextran sodium sulfate

[0044] In order to further study the scope of application of intestinal inflammation and the degree of inflammation development, with the method of the present invention, the test object is 17 days old healthy fast-growing hen and is randomly divided into 2 groups, each group is repeated 3 times, and each group repeats 16 Chickens, the control group drank regular water, and the treatment group drank 1.5% wt / vol DSS solution, and the test period was 16 days. The feeding method and management are the same as in Example 1.

[0045] Observation of experimental results:

[0046] During the test, the onset symptoms of the chickens were basically consistent with the 1.5% group in Example 1 in the first 10 days. After 10 days, the onset was accelerated, and severe blood in the stool and diarrhea occurred, and the weight loss was very obvious; The mortality rates of chickens in the tr...

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Abstract

The invention discloses a building method for a chicken enteritis model. The method comprises the following steps of (1) dissolving dextran sulfate sodium with a molecular weight of 36-50kDa in chicken drinking water to prepare 0.75-2.5% by weight of a dextran sulfate sodium solution, wherein the dextran sulfate sodium solution is used immediately after being prepared; (2) selecting a 14-18-day-age healthy quick-growth chicken as an object for building the chicken enteritis model; and (3) feeding the selected quick-growth chicken in a chicken house, and enabling the chicken to freely drink the dextran sulfate sodium solution prepared in the step (1) for more than 10 days. The building method for the chicken enteritis model is simple and convenient in operation; the effective dose range of the dextran sulfate sodium solution is wide, so that enteritis models of different pathological degrees can be built; and the method can be suitable for large-scale popularization and application in experiments.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chicken enteritis model induced by dextran sodium sulfate and a method for establishing the same. Background technique [0002] With the rapid development of high-density and intensive breeding production, the complexity of poultry intestinal diseases is becoming more and more obvious. At the same time, drug-resistant strains continue to emerge, which seriously threatens the health of chickens. The economic loss of the poultry industry due to coccidiosis is as high as 5 billion yuan every year in my country, and the drug alone is as high as 1.56-1.95 billion yuan (the average cost of anticoccidial drugs for each broiler is about 0.12-0.15 yuan). Dr. Gong from the Canadian Agricultural and Food Science Research Center pointed out at the 2014 International Symposium on Poultry Nutrition and Gut Health that the economic loss of the global poultry industry due to necrotic enteritis is ...

Claims

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Application Information

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IPC IPC(8): A61K31/716A61K49/00
CPCA61K31/716A61K49/0008
Inventor 邹娴计坚瞿浩罗成龙王劼郭福有
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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