Silica-based pH-sensitive fluorescent nanomaterials, preparation methods and applications thereof
A fluorescent nanomaterial and silica technology, which is applied in the field of biomedical materials, can solve the problems of poor stability and non-specific distribution of fluorescent dyes, and achieve the effects of cheap raw materials, high production efficiency and good stability.
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[0021] More specifically, the present invention discloses a method for preparing a silica-based fluorescent nanomaterial, comprising the following steps:
[0022] Step 1: Mix ethanol, ammonia water, and deionized water in a certain proportion in a container, such as a flask, and stir at 30°C for 0.5 to 1 hour;
[0023] Step 2: Quickly add a certain volume of silane reagent, continue to stir vigorously for 15 minutes to 24 hours, centrifuge and wash after the reaction, for example, centrifuge at 10,000 rpm, wash with ethanol three times, and finally ultrasonically disperse in ethanol;
[0024] Step 3: Disperse the fluorescent dye in dimethyl sulfoxide (DMSO), add it dropwise to the prepared silica nanoparticles, add different catalysts as needed to react for 0-24h, and finally through centrifugation or dialysis or ultra- The free dye was removed by filtration, and the particles were dispersed in deionized water or phosphate buffered saline solution with a pH of 7.4;
[0025] S...
Embodiment 1
[0037] (1) Measure 330mL of ethanol, 8mL of ammonia water and 110mL of deionized water in a round bottom flask and mix them at 30°C for 0.5h;
[0038] (2) Measure 20 mL of tetraethyl orthosilicate and aminopropyltriethoxysilane mixture (the volume ratio of the two is 9:1~0:10) and quickly add it to the mixed solution in step (1), Continue vigorous mechanical stirring for 15 min, centrifuge (10 000 rpm, 10 min) after the reaction, and wash 2-3 times with ethanol and deionized water respectively;
[0039] (3) Disperse the silica nanoparticles with amino groups on the obtained surface in a phosphate buffer solution with a pH of 8.0, and disperse the fluorescent dyes containing amino groups in DMSO solution, according to the molar ratio of amino groups of 10:1 to 2:1 Proportion Mix the solutions, and magnetically stir at room temperature for 0.5 h in the dark;
[0040](4) Add 50 μL of 0.25% glutaraldehyde solution dropwise to the mixed solution in step (3), and continue to stir a...
Embodiment 2
[0043] (1) Measure 330mL of ethanol, 8mL of ammonia water and 110mL of deionized water in a round bottom flask and mix them at 30°C for 0.5h;
[0044] (2) Measure 20 mL of tetraethyl orthosilicate and aminopropyltriethoxysilane mixture (the volume ratio of the two is 9:1~0:10) and quickly add it to the mixed solution in step (1), Continue vigorous mechanical stirring for 15 min, centrifuge (10 000 rpm, 10 min) after the reaction, and wash 2-3 times with ethanol and deionized water respectively;
[0045] (3) Disperse the obtained silica nanoparticles with amino groups on the surface in the phosphate buffer solution of pH 8.0, and disperse the fluorescent dyes containing aldehyde groups in DMSO solution, according to the amino group: aldehyde group molar ratio of 10: 1~ The solution was mixed at a ratio of 2:1, and the reaction was carried out under magnetic stirring at room temperature for 24 hours in the dark;
[0046] (4) Finally, the reaction solution was centrifuged at 10 ...
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