46results about How to "Good potential for clinical application" patented technology

The present invention relates to the removal of bilirubin from blood, and is especially cyclodextrin crossed polymer microsphere for adsorbing bilirubin and its preparation and application. The microsphere is prepared with diisocyanate as crosslinking agent and through condensation with cyclodextrin monomer. The polymer microsphere of the present invention has low cost and results in simplified experiment process, and the efficient removal of bilirubin with the polymer microsphere shows that the said material has excellent clinical application latent.

The invention discloses preparation of dendritic molecule-modifiedhydrophilic immunomagnetic beads and application of the hydrophilic immunomagnetic beads to rapid and efficient cell capture. The preparation method comprises the following steps: firstly, synthesizing magnetic nanoparticles through a hydrothermal method; hydrolyzing ethyl orthosilicate and modifying with a silylating reagent in order that the surface of a material is rich in amino groups; coupling dendritic molecules to the surface of the material in order that the material is hydrophilic; lastly, fixing an antibody to the surface of the material to prepare a capture substrate capable of recognizing tumor cells specifically. As proved by experiments, the hydrophilic immunomagnetic beads modified by the antibody and the dendritic molecules can shorten the time of combining the material and cells, perform high-specificity recognition and capture tumor cells rapidly, and capturing efficiency of 86+/-5 percent can be achieved only in 15 minutes; meanwhile, the activity of the cells is kept, and the tumor cells can be successfully separated from whole blood into which a tumor cell standard is added. A template-free, low-cost and low-toxicity material is applied to cell capture, so that the method is novel, convenient, practical and efficient, and has a tremendous clinical application potential.

The invention discloses a phenol recognition SERS probe, a preparation and an application thereof, and a universal ultra-sensitive immunoassay method based on SERS. Firstly, a phenol responsive SERS probe is prepared by reducing chloroauric acid with DTDBA, ahen, ELISA is combined with SERS, biomolecules are labeled with ALP, a substrate PPNA is hydrolyzed by ALP to generate phenol. An SERS probesignal caused by the phenol is used for realizing a technology for sensitive detection of the biomolecules. The technology can not only overcome the disadvantages of low sensitivity of the conventional enzyme linked immunosorbent assay, but also can solve the problems of enhancement and poor reproducibility of SERS detection signals. The phenol recognition SERS probe disclosed by the invention hasremarkable universality, can be widely used for immunoassay using the ALP as an enzyme label to measure a variety of biomolecules, lays a solid foundation for the development of immune technology based on SERS detection, and has a very large development space and a broad application prospect in the fields of biological and chemical detection, medical diagnosis, and the like.

The present invention relates to pleuromutilin antibiotic derivatives represented by a general formula (I), pharmaceutically acceptable salts, prodrugs, solvates or stereoisomers thereof, wherein R<2>, Q<1>, Q<2>, m and X are defined in an instruction. The present invention further relates to preparation methods of the compounds, drug compositions containing the compounds, drug preparations containing the compounds, and applications of the compounds in preparation of drugs for treatment and/or prevention of diseases caused by microorganisms.

The invention relates to the field of biological medicine, in particular to fusion protein of a cytokine combined chimeric antigen receptor and an application of the fusion protein. The fusion protein comprises a chimeric antigen receptor, 2A peptide, IL-7, 2A peptide and CCL3 which are sequentially connected in series, the chimeric antigen receptor comprises an scFv region, a hinge region, a transmembrane domain and an intracellular signal transduction region. According to the fusion protein of the cytokine combined chimeric antigen receptor and the application of the fusion protein, two cytokines IL-7 and CCL-3 are added during construction of a CAR vector, and the two cytokines can enhance the anti-apoptosis effect of CAR T cells and reduce the immunosuppression effect of the CAR T cells, so that the CAR T cells are helped to enhance the anti-tumor effect.

The invention discloses a metal organic framework composite nanomaterial as well as a preparation method and application thereof. The metal organic framework composite nano material comprises a functional material and a copper metal organic framework carrying the functional material, wherein the functional material comprises small organic molecules. The metal organic framework composite nanomaterial is good in chemical stability, has tumor microenvironment responsiveness, and can realize non-invasive, in-situ and efficient treatment and real-time monitoring of tumor parts.

The invention discloses an MMT (montmorillonite)-Gd-DTPA (diethylenetriaminepentaacetic acid) compound, a synthetic method thereof and an application of the MMT-Gd-DTPA compound to magnetic resonance diagnosis for a digestive tract. The synthetic method is characterized in that MMT powder and GdCl3 are mixed in water and react, MMT-Gd compound powder is obtained and reacts with DTPA in water, and the MMT-Gd-DTPA compound is obtained. The MMT-Gd-DTPA compound is obtained through hydro-thermal synthesis, the method is simple and easy to implement, the obtained compound not only has good longitudinal relaxation rate r1 and good biocompatibility, but also has good effect of adhesion to the digestive tract wall, is a better T1 magnetic resonance molecular imaging oral contrast agent and has higher clinical application potential.

The invention provides a lenvatinib (Len) liposome, a pharmaceutical composition thereof, a preparation method thereof and a prescription process optimization method. The liposome contains lenvatinib, phospholipid, an amphiphilic derivative, cholesterol and a gradient establishing substance, wherein the weight ratio of cholesterol to phospholipid is 1:2-1:10, the weight ratio of lenvatinib to phospholipid is 1:10-1:40, the weight ratio of the amphiphilic derivative to the phospholipid is 1:2-1:10, the phospholipid is glycerophospholipid or sphingomyelin, the gradient adopts a pH gradient method or an amine gradient method, and the concentration of the gradient establishing substance solution is 100-300mM. The Len liposome can actively target a tumor site through an SA-Siglec1 pathway, has the advantages of high encapsulation efficiency, high stability, high active targeting, high tumor inhibition rate and the like, and has good clinical application potential and product development value.

The invention discloses a hydroxychloroquine sulfate sustained-release microsphere for articular cavity injection and a preparation method thereof. The preparation method comprises the following steps: dissolving a surfactant in an organic solvent to form an oil phase; adding hydroxychloroquine sulfate and gelatin into water to obtain a water phase; dropwise adding the water phase into the oil phase, stirring and emulsifying to form a W/O type emulsion; transferring the W/O emulsion into an ice bath, and adding a curing agent for cross-linking curing; and then adding isopropanol for dehydration, suction filtration, washing and drying to obtain the hydroxychloroquine sulfate sustained-release microspheres for articular cavity injection. The prepared hydroxychloroquine microspheres can stably and continuously release drugs for more than 48 hours, a sustained-release preparation is obtained, the administration frequency can be reduced, the total dosage can be reduced, the compliance of patients can be improved, and the clinical application potential is good.

The present invention relates to a croton processing method for treatment of tuberculosis, and specific relates to a high temperature processing attenuation method of a traditional Chinese medicine croton and the role thereof in the treatment of tuberculosis. The croton processed product is obtained through three methods of high-temperature stir-frying, high temperature baking, and microwave heating. The processed croton has no toxic protein detected. LD50 increases significantly. The relative content of each component in the croton is essentially unchanged, and the croton oil content is relatively elevated. The processed croton is mixed with other traditional Chinese medicines to make pills which have significant role in resisting mycobacterium tuberculosis in vitro and in vivo. The croton processing method for treatment of tuberculosis disclosed by the invention has effects of reducing toxicity and enhancing efficacy, and has very prominent advantages compared with conventional croton processing methods such as baking, boiling, steaming, etc.

The invention discloses a methionine-polyesteramide high-molecular polymer with ROS responsiveness and application of the methionine polyesteramide high-molecular polymer. The structural formula of the methionine polyesteramide high-molecular polymer is shown as a formula (I). The methionine-based polyesteramide polymer material is synthesized by taking methionine, diacid containing different methylene numbers, diol and the like as raw materials through melt phase polycondensation reaction, the method is high in repeatability and simple and convenient to operate, the used raw materials are non-toxic, economical and easy to obtain, the obtained high-molecular polymer material has excellent biosafety and biodegradability, can be used as a drug carrier, can be controllably released in a high-concentration H2O2 environment, effectively solves the problems of poor stability, poor biosafety, weak targeting property and the like of nano-drugs, and has a good prospect in biomedical applications such as tumor treatment and the like.