Biological 3D printing ink and preparation method thereof

Abstract

The invention provides a biological 3D printing ink and a preparation method thereof. The preparation method comprises the following steps: S1: preparing a calcium hydroxide saturated solution, addinga silica nanoparticle suspension to the calcium hydroxide saturated solution, centrifuging and taking a precipitate to obtain modified nano-bioglass particles; S2: dissolving gelatin and sodium alginate in deionized water, then adding the modified nano-bioglass particles prepared in the Step S1, stirring evenly to prepare liquid hydrogel, sterilizing and equilibrating to obtain a gelatin-sodium alginate composite hydrogel; S3: dissolving solid calcium chloride powder in deionized water to prepare a calcium chloride solution, and sterilizing to obtain a cross-linking agent; and S4: adding thecross-linking agent to the gelatin-sodium alginate composite hydrogel for cross-linking to obtain the biological 3D printing ink. The biological 3D printing ink prepared by the invention has not onlysignificantly improved mechanical properties but also good gelling performance, rich pores and good cell-carrying potential.

Description

technical field [0001] The invention relates to the field of medical model manufacturing and application, in particular to a biological 3D printing ink and a preparation method thereof. Background technique [0002] The skin is the largest organ of the human body, which plays a very important role in maintaining the stability of the internal environment of the human body and the normal physiological functions of the body. Due to exposure to the external environment, skin damage and loss often occur. For small skin defects, the body Complete regeneration can be achieved to a certain extent through self-compensation, but for larger wounds, the skin generally undergoes scar repair, and for larger wounds, skin defects often delay healing or fail to heal. The body is directly exposed to the external environment, which not only fails to maintain the body’s fluid balance, but also exposes the patient to a high risk of infection. Therefore, it is very necessary to seal the wound in ...

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Problems solved by technology

[0004] Unlike the previous tissue engineering seed cells that migrate to the scaffold material and assist in promoting the microenvironment of regeneration, the cell-loaded bioink can integrate biomaterials, seed cells, and the regenerative microenvironment, which greatly improves the tissue engineered skin in the skin. The possibility of clinical application, and in the development of cell-loaded hydrogel, the selection and application of biomaterials and the appropriate addition of regenerative microenvironment are the focus of current research. Gelatin is due to its excellent biodegradability, biocompatibility, high Cell attachment and proliferation and low immunogenicity have been widely used in the research of wound dressings. In previous studies, gelatin-sodium alginate cell-loaded bioink not only has good biocompatibility, but also improves cell migration. , proliferation and differentiation, however, in the process of practical application, the gelatin-sodium alginate bioink printed with cell-loaded bioink has low hardness, resulting in a soft texture, which is difficult to apply to animal wound models

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