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Preparation method and application of biological ink containing PRP

A bio-ink and liquid water technology, applied in the field of 3D bio-printing, can solve the problems of scarless skin healing and lack of personalized functions of products, and achieve superior clinical application potential, good biosafety and biocompatibility Effect

Active Publication Date: 2021-12-24
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The skin is the largest organ of the human body, which plays a very important role in maintaining the homeostasis of the human body and normal physiological functions. Larger skin defects and pathological wounds (such as diabetic wounds) are difficult to achieve skin functionality through routine clinical disinfection and dressing changes. scarless healing
Although there are a variety of tissue engineered skins that can be used for wound treatment, these products lack personalized functions and cannot meet the needs of special wounds in clinical practice.

Method used

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  • Preparation method and application of biological ink containing PRP
  • Preparation method and application of biological ink containing PRP
  • Preparation method and application of biological ink containing PRP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Weigh 0.5g of gelatin and 0.05g of sodium alginate into a small beaker, add 4.5ml of PBS buffer solution, seal the beaker, dissolve in a water bath at 40°C for 30 minutes, and gently stir with a glass rod until the solution is uniform Without solute particles, the liquid hydrogel is obtained; the hydrogel is treated in a high-pressure steam sterilizer at 121°C for 30 minutes, and after the sterilization is completed, it is equilibrated at room temperature for 1 hour, and then sealed and refrigerated at 4°C;

[0028] Dissolve 2.5g of calcium chloride solid powder in 100ml of deionized water, stir magnetically until fully dissolved, and after high-pressure steam sterilization, seal and store at 4°C to obtain the crosslinking agent;

[0029] Mix 4.5ml of sterile gelatin-sodium alginate hydrogel, 0.375ml of PBS buffer and 0.125ml of PRP into a pre-filled syringe, and mix thoroughly with a sterile three-way device to obtain a PRP-containing bioink ( figure 1 No. 2 bioink); ...

Embodiment 2

[0033] Weigh 0.5g of gelatin and 0.05g of sodium alginate into a small beaker, add 4.5ml of PBS buffer solution, seal the beaker, dissolve in a water bath at 40°C for 30 minutes, and gently stir with a glass rod until the solution is uniform Without solute particles, the liquid hydrogel is obtained; the hydrogel is treated in a high-pressure steam sterilizer at 121°C for 30 minutes, and after the sterilization is completed, it is equilibrated at room temperature for 1 hour, and then sealed and refrigerated at 4°C;

[0034] Dissolve 2.5g of calcium chloride solid powder in 100ml of deionized water, stir magnetically until fully dissolved, and after high-pressure steam sterilization, seal and store at 4°C to obtain the crosslinking agent;

[0035] Mix 4.5ml of sterile gelatin-sodium alginate hydrogel, 0.25ml of PBS buffer and 0.25ml of PRP into a pre-filled syringe, and mix thoroughly with a sterile three-way device to obtain a PRP-containing bioink ( figure 1 No. 3 bioink);

...

Embodiment 3

[0038]Weigh 0.5g of gelatin and 0.05g of sodium alginate into a small beaker, add 4.5ml of PBS buffer solution, seal the beaker, dissolve in a water bath at 40°C for 30 minutes, and gently stir with a glass rod until the solution is uniform Without solute particles, the liquid hydrogel is obtained; the hydrogel is treated in a high-pressure steam sterilizer at 121°C for 30 minutes, and after the sterilization is completed, it is equilibrated at room temperature for 1 hour, and then sealed and refrigerated at 4°C;

[0039] Dissolve 2.5g of calcium chloride solid powder in 100ml of deionized water, stir magnetically until fully dissolved, and after high-pressure steam sterilization, seal and store at 4°C to obtain the crosslinking agent;

[0040] 4.5ml aseptic gelatin-sodium alginate hydrogel and 0.5mlPRP are packed in the prefilled syringe, utilize sterile three-way device to fully mix, promptly obtain the bioink containing PRP ( figure 1 No. 4 bioink);

[0041] Load the PRP-...

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Abstract

The invention provides a preparation method of biological ink containing PRP, and the preparation method comprises the following steps: preparing liquid hydrogel which comprises the following components in percentage by mass: 1%-30% of gelatin and 1%-10% of sodium alginate; fully mixing liquid hydrogel and PRP with the volume ratio being (9-36): 1 to be uniform, the quality standard of the PRP being as follows: based on the platelet concentration, the platelet concentration being larger than or equal to 1000 * 10<9> / L in a single platelet collection method, the platelet concentration being larger than or equal to 1 * 10<9> / L in a secondary centrifugation method, and obtaining the biological ink containing the PRP. The preparation method of the tissue organoid provided by the invention has the following advantages: (1) the bioactive components are added without changing the original physical and chemical properties of the bio-ink; and (2) the application safety and effectiveness in wound treatment are widely accepted, and excellent clinical application potential is achieved.

Description

technical field [0001] The invention relates to the field of biological 3D printing, in particular to a preparation method and application of a PRP-containing bio-ink. Background technique [0002] The skin is the largest organ of the human body, which plays a very important role in maintaining the homeostasis of the human body and normal physiological functions. Larger skin defects and pathological wounds (such as diabetic wounds) are difficult to achieve skin functionality through routine clinical disinfection and dressing changes. Heals without scarring. Although there are a variety of tissue engineered skins that can be used for wound treatment, these products lack personalized functions and cannot meet the needs of special wounds in clinical practice. Contents of the invention [0003] In order to solve the above technical problems, the present invention provides a preparation method of PRP-containing bio-ink, which can form a specific three-dimensional structure of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L5/04C08J3/075C08J3/24B33Y70/00
CPCC08J3/075C08J3/24B33Y70/00C08J2389/00C08J2405/04C08J2489/00
Inventor 宋薇李曌姚斌黄沙付小兵
Owner GENERAL HOSPITAL OF PLA
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