Tissue culture method of Glossostigma elatinoides

Abstract

A tissue culture method of Glossostigma elatinoides comprises the steps of acquiring and disinfecting an explant, cutting the explant into a stem with buds, inoculating to an initial medium, culturing to obtain adventitious buds, applying an ultrasonic field for 1-2 hours each during 1st to 7th days of initial culture, applying an ultrasonic field for 4-5 hours each day during 8th to 15th days of initial culture, transferring the adventitious buds to a subculture medium, culturing to obtain subculture seedlings, exposing to light for 10-12 hours per day under the temperature of 24+ / -2 DEG C and the humidity of 70-75% and under the light intensity of 2800-3200 lx, transferring the subculture seedlings to a rooting medium, culturing to obtain seedlings of Glossostigma elatinoides, and finally exercising and transplanting the seedlings; the rooting medium is made by adding ammonium molybdate, TDZ and amylopectin to N6 medium; the method is insusceptible to season, temperature and territory, mass aseptic seedlings are acquired, culture success rate of seedlings is increased, and the yield and quality are increased.

Description

technical field [0001] The invention relates to a tissue culture technique, in particular to a tissue culture method for dwarf pearls. Background technique [0002] Although dwarf pearls are aquatic plants with stems, the stems are not upright, but crawling and growing at the bottom. Because the creeping stolons will spread out in a diffuse shape, and finally cover the entire bottom, and the leaves on the stolons grow densely, which feels like a lawn. Generally, when planting dwarf pearls, in order to prevent the soft fine roots from being able to cling to the bottom sand, you should try to use finer sand in the choice of bottom sand. Dwarf pearls, barely 1 cm high, green creeping shoots with short root tissue, symmetrically grown spoon-shaped leaves 0.8 cm long and 0.3 cm wide, with blunt, slightly serrated tips, petioles almost as long as the leaves. Aerial leaf glands can produce short-stemmed white single-petal flowers, 0.3 cm in diameter, with 5 petals and 4 stamens. ...

AI Technical Summary

Problems solved by technology

However, the traditional seedling breeding method of dwarf pearls has reduced yield, serious virus accumulation, species degradation, greatly reduced quality and yield, and reduced planting benefits