Tissue culture method of Glossostigma elatinoides
A technology of tissue culture and dwarf pearls, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as quality and yield decline, planting benefit decline, and yield reduction, so as to improve yield and quality, and increase load capacity. Cultivate the survival rate and realize the effect of factoryization
Active Publication Date: 2017-02-22
浙江中日弹簧有限公司
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A tissue culture method of Glossostigma elatinoides comprises the steps of acquiring and disinfecting an explant, cutting the explant into a stem with buds, inoculating to an initial medium, culturing to obtain adventitious buds, applying an ultrasonic field for 1-2 hours each during 1st to 7th days of initial culture, applying an ultrasonic field for 4-5 hours each day during 8th to 15th days of initial culture, transferring the adventitious buds to a subculture medium, culturing to obtain subculture seedlings, exposing to light for 10-12 hours per day under the temperature of 24+/-2 DEG C and the humidity of 70-75% and under the light intensity of 2800-3200 lx, transferring the subculture seedlings to a rooting medium, culturing to obtain seedlings of Glossostigma elatinoides, and finally exercising and transplanting the seedlings; the rooting medium is made by adding ammonium molybdate, TDZ and amylopectin to N6 medium; the method is insusceptible to season, temperature and territory, mass aseptic seedlings are acquired, culture success rate of seedlings is increased, and the yield and quality are increased.
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