Tissue culture method of Glossostigma elatinoides

A technology of tissue culture and dwarf pearls, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as quality and yield decline, planting benefit decline, and yield reduction, so as to improve yield and quality, and increase load capacity. Cultivate the survival rate and realize the effect of factoryization

Active Publication Date: 2017-02-22
浙江中日弹簧有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional seedling breeding method of dwarf pearls has reduced yield, serious virus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0025] Example 1

[0026] The tissue culture method of Dwarf Pearl includes the following steps:

[0027] (1) Obtain and disinfect the explant

[0028] Choose new shoots of robust plants, wash the dirt and dust on the surface of the shoots with tap water, first soak in alcohol, then disinfect with mercury, and finally rinse with sterile water to obtain disinfected explants.

[0029] (2) Primary training

[0030] Divide the sterilized explants into shoots with buds, then put them into the primary culture medium and cultivate them for 32 days to obtain adventitious buds.

[0031] The initial culture conditions are as follows: temperature is 22℃, humidity is 63%, illumination time is 8 hours / day, light intensity is 22001x, first to 7 days of primary culture, ultrasonic field is applied for 1 hour every day, ultrasonic field power is 8W / cm 2 , The ultrasonic field frequency is 20KHz, and the ultrasonic field is applied for 4 hours every day on the 8th to 15th day of the primary culture, and ...

Example Embodiment

[0043] Example 2

[0044] The tissue culture method of Dwarf Pearl includes the following steps:

[0045] (1) Obtain and disinfect the explant

[0046] Choose new shoots of robust plants, wash the dirt and dust on the surface of the shoots with tap water, first soak in alcohol, then disinfect with mercury, and finally rinse with sterile water to obtain disinfected explants.

[0047] (2) Primary training

[0048] Divide the sterilized explants into shoots with buds, then put them into the primary culture medium and cultivate them for 36 days to obtain adventitious buds.

[0049] The initial culture conditions are as follows: temperature is 26℃, humidity is 68%, light time is 10 hours / day, light intensity is 25001x, first to 7 days of primary culture, ultrasonic field is applied for 2 hours every day, ultrasonic field power is 12W / cm 2 , The ultrasonic field frequency is 22KHz, and the ultrasonic field is applied for 5 hours every day on the 8th to 15th day of the primary culture, and the ...

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PUM

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Abstract

A tissue culture method of Glossostigma elatinoides comprises the steps of acquiring and disinfecting an explant, cutting the explant into a stem with buds, inoculating to an initial medium, culturing to obtain adventitious buds, applying an ultrasonic field for 1-2 hours each during 1st to 7th days of initial culture, applying an ultrasonic field for 4-5 hours each day during 8th to 15th days of initial culture, transferring the adventitious buds to a subculture medium, culturing to obtain subculture seedlings, exposing to light for 10-12 hours per day under the temperature of 24+/-2 DEG C and the humidity of 70-75% and under the light intensity of 2800-3200 lx, transferring the subculture seedlings to a rooting medium, culturing to obtain seedlings of Glossostigma elatinoides, and finally exercising and transplanting the seedlings; the rooting medium is made by adding ammonium molybdate, TDZ and amylopectin to N6 medium; the method is insusceptible to season, temperature and territory, mass aseptic seedlings are acquired, culture success rate of seedlings is increased, and the yield and quality are increased.

Description

technical field [0001] The invention relates to a tissue culture technique, in particular to a tissue culture method for dwarf pearls. Background technique [0002] Although dwarf pearls are aquatic plants with stems, the stems are not upright, but crawling and growing at the bottom. Because the creeping stolons will spread out in a diffuse shape, and finally cover the entire bottom, and the leaves on the stolons grow densely, which feels like a lawn. Generally, when planting dwarf pearls, in order to prevent the soft fine roots from being able to cling to the bottom sand, you should try to use finer sand in the choice of bottom sand. Dwarf pearls, barely 1 cm high, green creeping shoots with short root tissue, symmetrically grown spoon-shaped leaves 0.8 cm long and 0.3 cm wide, with blunt, slightly serrated tips, petioles almost as long as the leaves. Aerial leaf glands can produce short-stemmed white single-petal flowers, 0.3 cm in diameter, with 5 petals and 4 stamens. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 不公告发明人
Owner 浙江中日弹簧有限公司
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