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Aurantia II degrading bacteria AO7-1 and microbial agent produced from same

A technology for degrading bacteria and inoculants, applied in the biological field, can solve problems such as relatively mature, stable and efficient technology, comprehensive management of polluted environment, harm to human health, etc., so as to protect human health, protect the ecological environment, produce Low cost of use

Active Publication Date: 2017-02-22
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The azo dye Golden Orange II product is soluble in water. The environmental pollution of the azo dye Golden Orange II has seriously endangered human health. There is no relatively mature, stable and efficient technology for the comprehensive treatment of its polluted environment.

Method used

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  • Aurantia II degrading bacteria AO7-1 and microbial agent produced from same
  • Aurantia II degrading bacteria AO7-1 and microbial agent produced from same
  • Aurantia II degrading bacteria AO7-1 and microbial agent produced from same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Production Example 1: Production of golden orange II degrading bacteria AO7-1 inoculum according to the following steps

[0033] Step (1): Scrape a loop of golden orange II degrading bacteria AO7-1 with an inoculation loop and inoculate it in the inoculation, 30°C, 180rpm shaking culture to logarithmic phase; wherein, the weight of each component in 1L fermentation medium The content is: peptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the rest deionized water, pH 9.0;

[0034] Step (2): Inoculate the cultured bacteria in step (1) into a 500L seed tank at 1% of the volume of the culture medium, and cultivate to the logarithmic growth phase; the weight content of each component in the 1L seed tank culture medium is: peptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the rest deionized water, pH 9.0; the culture conditions are: sterile air is passed through during the cultivation process, and the ratio of sterile air to the volume of the culture medium is 1:0.6, stirr...

Embodiment 2

[0037] Production Example 2: Production of Golden Orange II degradation bacteria AO7-1 inoculum according to the following steps

[0038] Step (1): Scrape a loop of golden orange II degrading bacteria AO7-1 with an inoculation loop and inoculate it in the inoculation, 30°C, 180rpm shaking culture to logarithmic phase; wherein, the weight of each component in 1L fermentation medium The content is: peptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the rest deionized water, pH 9.0;

[0039] Step (2): Inoculate the cultured bacteria in step (1) into a 500L seed tank at 1% of the volume of the culture medium, and cultivate to the logarithmic growth phase; the weight content of each component in the 1L seed tank culture medium is: peptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the rest deionized water, pH 9.0; the culture conditions are: sterile air is passed through during the cultivation process, and the ratio of sterile air to the volume of the culture medium is 1:0.9, sti...

Embodiment 3

[0042] Production Example 3: Production of golden orange II degrading bacteria AO7-1 inoculum according to the following steps

[0043] Step (1): Scrape a loop of golden orange II degrading bacteria AO7-1 with an inoculation loop and inoculate it in the inoculation, 30°C, 180rpm shaking culture to logarithmic phase; wherein, the weight of each component in 1L fermentation medium The content is: peptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the rest deionized water, pH 9.0;

[0044] Step (2): Inoculate the cultured bacteria in step (1) into a 500L seed tank at 1% of the volume of the culture medium, and cultivate to the logarithmic growth phase; the weight content of each component in the 1L seed tank culture medium is: peptone 1%, yeast extract 0.5%, sodium chloride 0.5%, the rest deionized water, pH is 9.0; the culture conditions are: sterile air is passed through during the cultivation process, and the ratio of sterile air to the volume of the medium is 1:1.2, stirring s...

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Abstract

The invention discloses aurantia II degrading bacteria AO7-1 and a microbial agent produced from the same. A strain of the aurantia II degrading bacteria AO7-1 is Pseudochrobactrum saccharolyticum, and the strain AO7-1 is preserved in the CCTCC (China Center for Type Culture Collection) in Wuhan University, Wuhan, China on September 19, 2016 and has the preservation number of CCTCC M2016501. The optimum growth temperature for the bacteria is about 30 DEG C, the optimum environment pH is about 9.0, the residual quantity of azo dyes aurantia II can be reduced by 90% or above by means of the degrading bacteria AO7-1, and the microbial agent has the advantages of being low in production and use cost, convenient to use and good in removal effect and is suitable for biological enhancement treatment of related dye wastewater and pollution treatment of water or soil.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the golden orange II degrading bacteria AO7-1 and the produced bacterial agent. The method uses microorganisms to degrade the azo dye golden orange II and is suitable for the treatment of environmental pollution. Background technique [0002] Azo dyes (a type of compound with aryl groups connected at both ends of the azo group) are the most widely used synthetic dyes in the printing and dyeing process of textiles and clothing. They are used for the dyeing and printing of a variety of natural and synthetic fibers, as well as paints. , Plastic, rubber, etc. Under special conditions, it can decompose to produce more than 20 kinds of carcinogenic aromatic amines, which can change the structure of human DNA through activation to cause pathological changes and induce cancer. [0003] The common feature of azo compounds is that they contain an azo bond "-N=N-" in the molecule. General...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 张迹袁巧云杨威王新风
Owner HUAIYIN TEACHERS COLLEGE
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