Key microbial functional genome detection method in pepper peeling process

Abstract

The invention discloses a key microbial functional genome detection method in a pepper peeling process. According to the key microbial functional genome detection method in the pepper peeling process, from the perspective of micro-ecosystem, pepper epidermis microorganisms in the peeling process are taken as an object; metagenome DNA of pepper epidermis bacteria is extracted and purified and a variable region sequence is amplified, and then the variable region sequence of the epidermis bacteria is read with the application of a high-throughput sequencing technology; by virtue of a bioinformatics platform, the sequence is subjected to quality control and species annotation and function prediction are completed, and on the basis, a microorganism species, which plays a vital role in the pepper peeling process, in fresh fruit epidermis and a core metabolic pathway thereof are comprehensively and objectively revealed depending on a statistical method. The detection method provided by the invention simplifies experimental operations and improves a sequencing efficiency, so that the development of metagenomics is greatly promoted.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a method for detecting functional genomes of key microorganisms in the pepper peeling process. Background technique [0002] Pepper was first recorded in "Tang Materia Medica" compiled by Su Jing and others during the Tang Xianqing period. It is a perennial evergreen vine of the Piperaceae family. Pepper is suitable for growing in humid, high temperature, windless and fertile soil areas, so it is most widely planted in areas within 500m above sea level near the north and south latitudes of the equator. As the most important tropical spice crop in the world, pepper was first produced in India. With the promotion and planting of pepper, the crop is loved by people all over the world and has a wide range of uses in the fields of medicine and food. In the Ming Dynasty, Li Shizhen once mentioned in "Compendium of Materia Medica": "Pepper, because it is pungent...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for detecting the functional genome of key microorganisms in the peeling process of pepper, aiming to solve the problem that the primary processing of pepper peeling in my country still stays at the level of the traditional dipping method, and the quality of the obtained pepper is low, not only the color is gray, And there is a serious odor problem

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