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Method of simultaneously detecting contents of vitamin A and vitamin E in blood

A vitamin and content technology, applied in the field of vitamin detection, can solve the problems of inaccurate qualitative and quantitative, low sensitivity, and many interference factors, and achieve the effects of improving sample recovery, short analysis time, and accurate quantitative results

Active Publication Date: 2017-02-22
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The previous methods have the problems of complicated pre-processing, many interference factors, long analysis time, low sensitivity, and inaccurate qualitative and quantitative analysis.

Method used

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  • Method of simultaneously detecting contents of vitamin A and vitamin E in blood
  • Method of simultaneously detecting contents of vitamin A and vitamin E in blood
  • Method of simultaneously detecting contents of vitamin A and vitamin E in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A method for simultaneously detecting the content of vitamin A and vitamin E in blood, using liquid chromatography for detection, comprising the steps of:

[0067] (1) Centrifuge the whole blood at a speed of 3500r / min and a centrifugation time of 10min, take the supernatant to obtain serum, and store the serum at 4°C-8°C in a dark place (within 24h) until analysis For use, or store in -20°C under dark conditions (avoid repeated freezing and thawing) (within 72h) until analysis, or store in -80°C under dark conditions (avoid repeated freezing and thawing) (within 30 days) ) to be used before analysis;

[0068] (2) Calibration of standard solution

[0069] Mix 90ul of the standard working solution of six different concentrations of standard products with 10ul of the internal standard working solution and vortex mix at 1400r / min for 30 seconds, mix well, and make a standard curve series of solutions; the standard curve series of solutions must be Carry out the UV spectr...

Embodiment 2

[0105] A method for simultaneously detecting the content of vitamin A and vitamin E in blood, using liquid chromatography tandem mass spectrometry for detection, comprising the following steps:

[0106] (1) Centrifuge the whole blood at a speed of 3500r / min and a centrifugation time of 10min, take the supernatant to obtain serum, and store the serum at 4°C-8°C in a dark place (within 24h) until analysis For use, or store in -20°C under dark conditions (avoid repeated freezing and thawing) (within 72h) until analysis, or store in -80°C under dark conditions (avoid repeated freezing and thawing) (within 30 days) ) to be used before analysis;

[0107] (2) Calibration of standard solution

[0108] Mix 90ul of the standard working solution of six different concentrations of standard products with 10ul of the internal standard working solution and vortex mix at 1400r / min for 30 seconds, mix well, and make a standard curve series of solutions; the standard curve series of solutions ...

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Abstract

The invention discloses a method of simultaneously detecting contents of vitamin A (retinol) and vitamin E (alpha-tocopherol) in blood. The method comprises the following steps: (1) centrifuging a whole blood sample, and taking a supernatant to obtain serum or plasma for standby application; (2) calibrating a standard solution; (3) pretreating the sample; and (4) automatically putting 80 microlitres of the supernatant sample in the step (3) in a sample bottle, analyzing through liquid chromatography or liquid chromatography-tandem mass spectrometry, and simultaneously quantitatively detecting the contents of the vitamin A (retinol) and the vitamin E (alpha-tocopherol) in the blood. The method of simultaneously detecting the contents of the vitamin A (retinol) and the vitamin E (alpha-tocopherol) in the blood is strong in specificity, high in accuracy and sensitivity and short in analysis time.

Description

technical field [0001] The invention relates to the technical field of vitamin detection, in particular to a method for simultaneously detecting the contents of vitamin A and vitamin E in blood. Background technique [0002] Vitamin A (VA) is a fat-soluble alcohol substance with various molecular forms. It is the first vitamin to be discovered. Among them, vitamin A1 (VA1) mainly exists in animal liver, blood and retina of the eye, also known as retinol. , melting point 64°C, molecular formula C 20 h 30 O, the chemical structural formula of vitamin A1 (retinol) is shown in structural formula (1); vitamin A1 (VA2) mainly exists in freshwater fish, and its melting point is only 17-19 ° C. 20 h 28 O, also known as retinal. Vitamin A is a component of rhodopsin that senses weak light in visual cells. The lack of vitamin A in the human body affects the ability to adapt to the dark, such as children with stunted growth, dry skin, dry eye disease, night blindness, and age spots...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 倪君君李玮
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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