Unlock instant, AI-driven research and patent intelligence for your innovation.

A method and device for collecting soil denitrifying microorganisms to produce nitrogen

A denitrification microorganism and soil technology, applied in the field of soil denitrification microorganism science, can solve problems such as difficult, quantitative research and reduction, and achieve the effect of accurate collection

Inactive Publication Date: 2018-11-20
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the air contains about 78% N 2 , so it is necessary to accurately collect the N produced by denitrifying microorganisms 2 It is very difficult, which makes it impossible to quantitatively study how much nitrous oxide gas is reduced to nitrogen by denitrifying microorganisms. At present, no methods and devices have been reported in this regard.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method and device for collecting soil denitrifying microorganisms to produce nitrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for collecting soil denitrifying microorganisms to produce nitrogen, the steps of which are:

[0027] 1) Put the soil to be studied into a sealed culture bottle equipped with a first three-way valve: accurately weigh 100 g of the soil to be studied with a water content of 60%, and place it at the bottom of the culture bottle (with a three-way cap on the bottle cap) through an iron spoon. ) to ensure that the soil is even on the bottom of the bottle.

[0028] 2) Put the culture bottle containing the soil, the needle with the needle cover, and the N 2 Put O air bag (with tee) etc. into the vacuum bag 1, press the zipper slider 6 with your hands, and slowly and lightly pull to the other end of the zipper 5 to seal the vacuum bag. Use an external vacuum pump to connect the air nozzle port 4 to vacuumize the vacuum bag;

[0029] 3) Open the second three-way valve connected to the He gas tank, and the third three-way valve 2 of the PU pipe outside the vacuum bag, a...

Embodiment 2

[0033] according to figure 1 It can be seen that a method of collecting soil denitrifying microorganisms to produce N 2The device consists of vacuum bag 1, third three-way valve 2, fourth three-way valve 8, PU tube 3, air nozzle 4, zipper 5, zipper slider 6, first rubber glove 7, second rubber glove 9 composition. It is characterized in that: the vacuum bag 1 is composed of two reinforced PA+PE materials of 80×65cm, the two long sides and the first short side 10 of the two materials are bonded by Jinghong H-1502PP polypropylene special glue, and the two materials are two The long side and the second short side 11 fix the two parts of the zipper 5 with H-1502PP polypropylene special glue respectively, and the zipper slider 6 is embedded on the zipper 5 . One end of the PU tube 3 is in the vacuum bag 1, and the other end is outside the vacuum bag 1. The PU tube 3 is 80 cm long, 40 cm is in the vacuum bag 1, and 40 cm is outside the vacuum bag 1. The distance between the vacuum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method and device for collecting nitrogen produced by soil denitrifying microorganisms. The steps are: 1) Put the soil to be studied into a sealed culture bottle equipped with a three-way valve; 2) Put the soil into the culture bottle and needle , syringes, N2O gas bags, gas bottles, etc. are put into the vacuum bag, then the vacuum bag is sealed, and the vacuum bag is evacuated using a vacuum pump; 3) Open the three-way valve connected to the gas tank, and fill the vacuum bag with He gas, so that the vacuum bag expands and has a certain operating space; 4) After evacuating the sample culture bottle, fill it with N2O; 5) After the reaction for a certain period of time, use a syringe to collect the reacted gas from the culture bottle and wait in the gas cylinder. Measurement. It consists of a vacuum bag body, a three-way valve, a PU pipe, an air nozzle, a zipper, a zipper slider, and rubber gloves. This method is simple, easy to operate, can accurately collect N2 produced by soil denitrifying microorganisms, has a simple structure, is easy to use, and can effectively avoid contamination of samples by external nitrogen during the gas collection process.

Description

technical field [0001] The invention relates to the field of soil denitrification microorganism science and technology, more specifically to a device for producing nitrogen by soil denitrification microorganisms, and also to a method for producing nitrogen by soil denitrification microorganisms, which is used to study denitrification microorganisms or denitrification in soil The ability of pure bacteria to reduce nitrous oxide to nitrogen. Background technique [0002] The process of soil denitrification is the atmospheric N 2 Major source of O and other nitrogen oxides (Byrnes 1990). The process of soil denitrification has a significant impact on the environment. Denitrification intermediate product N 2 O is not only an important greenhouse gas, but also destroys the atmospheric stratosphere O 3 Layer, Ravishankara of the US National Oceanic and Atmospheric Administration pointed out in Science, N 2 The emission of O is the most important single greenhouse gas that des...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/24
CPCG01N33/24G01N33/245
Inventor 刘春梅侯海军张文钊刘毅魏文学
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI