Preparation method for hair keratin-cell tissue composite engineered dressing

A technology of cell tissue and keratin, applied in medical science, absorbent pads, bandages, etc., can solve problems such as repairing wounds, and achieve the effects of accelerating wound healing, non-immunogenicity, and non-toxic side effects

Inactive Publication Date: 2017-03-08
北京东方艾美生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly treated by western medicine, and there is no clinically used biomaterials to repair wounds.

Method used

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  • Preparation method for hair keratin-cell tissue composite engineered dressing
  • Preparation method for hair keratin-cell tissue composite engineered dressing
  • Preparation method for hair keratin-cell tissue composite engineered dressing

Examples

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preparation example Construction

[0047] A preparation method of an engineered dressing, the preparation method comprising the preparation of hair keratin, the preparation of the cell suspension and the preparation of the dressing, the specific method is as follows:

[0048] (1) Preparation of hair keratin:

[0049] S1: Degreasing and softening: Weigh the hair for degreasing and softening;

[0050] S2: Decolorization: put the softened hair in a decolorization solution for decolorization, the composition of the decolorization solution is 3-5 parts by weight of sodium pyrophosphate, 1-2 parts by weight of potassium persulfate, 70-90 parts by volume of hydrogen peroxide, concentrated 22-33 parts by volume of ammonia water and 350-450 parts by volume of water;

[0051] S3: Cleaning and drying: Soak the decolorized hair in water, rinse with water after soaking, filter, and dry overnight at 37°C in a blast drying oven

[0052] S4: Powder preparation: cut the dried hair into pieces, and add liquid nitrogen to freez...

Embodiment 1

[0069] A preparation method of an engineered dressing, the preparation method comprising the preparation of hair keratin, the preparation of the cell suspension and the preparation of the dressing, the specific method is as follows:

[0070] (1) Preparation of hair keratin:

[0071] S1: Degreasing: Weigh 5g of hair and put it in a 1000ml beaker. Add 99.7% absolute ethanol to soak for 30 minutes, stir once with a glass rod every 5 minutes, and rinse with running water;

[0072] S2: Softening: Place the washed hair in a solution of ≥10% sodium hypochlorite 10ml and distilled water 90ml, soak at room temperature for 30 minutes, stir with a glass rod once every 5 minutes, and rinse with running water;

[0073] S3: decolorization: by the weight of 5g hair, take by weighing 99% sodium pyrophosphate (Na 4 P 2 o 7 )4g, 99.5% potassium persulfate (K 2 S 2 o 8 ) 1.5g, 30% hydrogen peroxide (H 2 o 2 ) 80ml, 25% concentrated ammonia water 27.5ml, distilled water 400ml, and make i...

Embodiment 2

[0088] The preparation method (as embodiment 1) of hair keratin among the present invention can also be replaced by following method:

[0089] Add water to the high-purity keratin mud obtained after sterilization in step S9 in Example 1, mix well, place in an incubator and incubate for 2-3 hours until a relatively uniform color and character appear; sterilize to obtain gelled hair keratin;

[0090] Then the gelled hair keratin is compounded with the cell suspension of the present invention to make the engineering dressing of the present invention. Example: Contains 5×10 5 Fbs is composed of 200ul PBS mixed with 200ul gel hair keratin.

[0091] Adopt this method and the raw material of the method in embodiment 1 is identical, and effect and the effect that reach are all similar, can replace each other.

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Abstract

The invention relates to a preparation method for a hair keratin-cell tissue composite engineered dressing. The preparation method comprises the following steps: preparation of hair keratin; preparation of a cell suspension; and preparation of the dressing. The engineered dressing provided by the invention is a wound covering material with excellent effect, is capable of efficiently preventing bacterial infection, accelerating wound healing and has the advantages of good biocompatibility, capacity of controlling and absorbing exudates from wounds and allowing permeation of gas and water vapor, good air permeability, protection effect on newborn tissue, no toxic and side effect, etc. More importantly, the engineered dressing can be dissolved and absorbed by the body, so considerable bleeding and pains of patients during removing of the engineered dressing are avoided, no scrap is left to delay wound healing, and an environment beneficial for wound healing is provided for promotion of skin regeneration.

Description

technical field [0001] The invention relates to a dressing, in particular to a preparation method of a hair keratin composite cell tissue engineering dressing applied to a wound. Background technique [0002] Human and animal trauma can be seen everywhere, and all kinds of trauma can cause cell degeneration, necrosis and tissue defect in different degrees, and tissue repair must be carried out through cell proliferation and formation of intercellular matrix. At present, it is mainly treated by western medicine, and there is no clinically used biomaterials to repair wounds. [0003] Fibroblasts are the main repair cells in wound healing, which activate, proliferate, and synthesize collagen in the process of wound repair. This application intends to use keratin biomaterials as a scaffold system, compound fibroblasts, construct tissue-engineered dressings that simulate body tissues and functions, rebuild or restore skin barriers, and form wound coverings with biological dressi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/40A61L15/64A61L15/42
CPCA61L15/32A61L15/40A61L15/42A61L15/64C08L89/00
Inventor 张娇张飞
Owner 北京东方艾美生物技术股份有限公司
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