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a frozen rod

A technology of carrying rods and slides, applied in the field of medical devices, can solve the problems of uneven surface tension, increased thawing time, easy falling of slides, etc., achieves strong hygroscopicity and acid and alkali resistance, avoids cross infection, good The effect of saving the environment

Active Publication Date: 2017-08-04
BEIJING ZHONGYI KANGWEI MEDICAL INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned method of carrying embryos has the following defects: first, the slides are directly glued to the glass rod with glue, and the slides are easy to fall during storage; second, the original carrier is the latest plastic carrier. The surface tension is not uniform, and the embryo will appear spherical or flattened during use, which is not conducive to observation and is not conducive to absorbing the freezing liquid around the embryo. If it is directly frozen in this way, the thawing time will be increased during thawing, which will affect the embryo. quality; thirdly, there is no fixed place to place the embryo on the longer carrier, but placed on a certain position on the slide according to the doctor's habit. When observing, it is necessary to find the position of the embryo. The sheath will have the risk of knocking down the embryos; fourth, several people's embryos will be placed in a large liquid nitrogen bucket, and the liquid nitrogen is not used for one-time use, and there will be a risk of virus cross-infection

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] The arc rad of the slide 3 is 2.12°, and the thickness d is 0.2 mm. When embryos need to be frozen, the embryo droplet is placed on the curved slide 3, and then the holding rod part is removed from the handle 1 Place the outside into the cylindrical hollow tube 4 of the outer tube of the carrier rod, and then place the frozen carrier rod in the liquid nitrogen tank. The frozen carrier rod is under the action of the steel ring 5 to make the frozen carrier rod sink quickly in the liquid nitrogen. Shorten its sinking time, and let the embryo freezing carrier fully contact with liquid nitrogen for cryopreservation. During the storage process, the 2.12° arc-shaped slide 3 can ensure that the frozen droplets are in a three-dimensional shape and ensure that the frozen droplets can Better freezing.

Embodiment 2

[0032] The arc rad of the slide 3 is 2.32°, and the thickness d is 0.25 mm. When embryos need to be frozen, the embryo droplet is placed on the curved slide 3, and then the holding rod part is removed from the handle 1 Place the outside into the cylindrical hollow tube 4 of the outer tube of the carrier rod, and then place the frozen carrier rod in the liquid nitrogen tank. The frozen carrier rod is under the action of the steel ring 5 to make the frozen carrier rod sink quickly in the liquid nitrogen. Shorten its sinking time and allow the embryo freezing carrier to fully contact with liquid nitrogen for cryopreservation. During the preservation process, the 2.32° arc-shaped slide 3 can fit the droplet under natural weight. The surface curvature maintains the three-dimensional shape of the frozen droplets, so that the frozen liquid around the sample in the frozen droplets is evenly distributed, ensuring that the frozen droplets are uniformly frozen.

Embodiment 3

[0034] The arc rad of the slide 3 is 2.52°, and the thickness d is 0.3mm. When embryos need to be frozen, the embryo droplet is placed on the curved slide 3, and then the holding rod part is removed from the handhold 1 Place the outside into the cylindrical hollow tube 4 of the outer tube of the carrier rod, and then place the frozen carrier rod in the liquid nitrogen tank. The frozen carrier rod is under the action of the steel ring 5 to make the frozen carrier rod sink quickly in the liquid nitrogen. The sinking time is shortened, and the freezing rod containing the embryo is fully contacted with liquid nitrogen for cryopreservation. During the preservation process, the 2.52° arc-shaped slide 3 can keep the droplet in a three-dimensional shape under natural weight.

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Abstract

The invention relates to a refrigeration loading rod, and belongs to the technical field of medical instruments. The refrigeration loading rod comprises a loading rod part and a loading rod outer cannula. The loading rod part disclosed by the invention is integrally formed, so as to prevent a slide glass from dropping out; the radian of the designed arc-shaped slide glass can fit for the radian of the surface of a drop under the natural gravity, so that the three-dimensional shape of the refrigerating drop is kept, and refrigerating fluid around a sample in the refrigerating drop can be uniformly distributed, so as to ensure that the refrigerating fluid is uniformly refrigerated; after the slide glass loaded with embryos is loaded in a cylindrical hollow tube, the refrigeration loading rod can quickly sink in liquid nitrogen under the action of a steel ring, so that the refrigeration loading rod can be in full contact with the liquid nitrogen; since the part of the slide glass, which is loaded with the embryos, is completely located in the cylindrical hollow tube, and a cotton plug for fixing a powder plug is formed by weaving synthetic fibers in specific proportion, the air permeability of the embryos is ensured, the moisture absorption and the resistance to acid and base are stronger, a good storage environment is provided for the embryos, and the cross infection of the embryos in a liquid nitrogen pot is effectively avoided.

Description

Technical field [0001] The invention belongs to the technical field of medical devices, and specifically relates to a freezing carrier rod. Background technique [0002] With the increase of infertility population, more and more people are receiving embryo transfer. In open vitrification, embryos are in direct contact with liquid nitrogen, which makes the toxic substances in liquid nitrogen potentially dangerous to embryos, so many In vitro fertilization-embryo transfer (IVF) laboratories use enclosed vitrification as the main method for storing embryos, eggs, blastocysts, etc. [0003] However, the embryo-carrying carrier is relatively simple and rough at present. For example, the embryo-carrying carrier is directly glued to the glass rod with glue, or the glass rod is melted and pulled into a thin sheet, and the embryo is placed on it; or the embryo is carried by a plastic tube. Embryos, the latest product is to put embryos on a plastic sheet and then cover with a cap, or direct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0242
Inventor 费嘉冯涛
Owner BEIJING ZHONGYI KANGWEI MEDICAL INSTR
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