Special microbial organic fertilizer for leguminous crops, preparation method of fertilizer and application
A technology of microbial organic fertilizer and leguminous crops, which is applied in the preparation of organic fertilizers, the treatment of biological organic parts, organic fertilizers, etc. It can solve the problems of increased drug resistance of crop pathogens, affecting the health of food people, and the reduction of organic matter in cultivated land. , to reduce stress and agricultural production costs, improve utilization, and assist in the absorption of nutrients
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[0038] The preparation of 1 rhizobia bacterial agent:
[0039] A. slope culture: inoculate the original species of rhizobium on the slope culture medium under aseptic operation, and cultivate it for 4-5d at 28°C;
[0040] B. shaker culture: the slant bacterial classification cultivated in step a is eluted in the seed culture medium with sterile water, 28-30 ℃, 120-140r / min shaker culture 20-24h;
[0041] c. Fermentation tank culture:
[0042] Inoculate the bacteria cultured in step b into the liquid fermentation medium under aseptic operation, the inoculum size is 2-5%, the rotation speed is 180-200r / min, the culture temperature is 25-28°C, the ventilation rate is 1:0.6-0.8vvm, and culture 3-5 days, stop fermentation, add the sodium molybdate of 2.5-5% weight in fermented liquid; With the centrifugation of gained fermented liquid through butterfly centrifuge, 3000r / min, collect the filter residue after centrifuging, add certain amount (according to bacterium agent requirements...
Embodiment 1
[0055] Embodiment 1 special microbial organic fertilizer for leguminous crops and preparation method thereof
[0056] This embodiment provides a special microbial organic fertilizer for leguminous crops, which is formed by composting and fermenting the fermentation product of the rice straw mixture using a composite functional bacterial starter containing Rhizobium and Rhodopseudomonas palustris; the rice straw mixture The fermented product is made by fermenting the mixture of rice straw with the fermentation agent containing Bacillus subtilis and Trichoderma harzianum; the mixture of rice straw includes the following raw materials in parts by weight: 30 parts of rice straw, 45 parts of pig manure, 18 parts of rapeseed meal wherein the amount of fermentation agent used in the preparation process of the fermentation product of the rice straw mixture is 5 parts; in the fermentation agent: the number of viable bacteria of Bacillus subtilis is 1.2 billion CFU / g, and the number of v...
Embodiment 2
[0066] Embodiment 2 leguminous crop special microbial organic fertilizer and preparation method thereof
[0067] This example provides a special microbial organic fertilizer for leguminous crops. The only difference from Example 1 is that the rice straw mixture includes the following raw materials in parts by weight: 15 parts of rice straw, 35 parts of pig manure, and 5 parts of rapeseed meal .
[0068] This example also provides the preparation method of the above-mentioned special microbial organic fertilizer for leguminous crops. The difference from Example 1 is that the first fermentation time is 20 days, and the second fermentation time is 10 days.
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