A kind of cucumber seedling raising substrate and preparation method thereof
A seedling raising substrate and cucumber technology, applied in the preparation, application, and waste fuel of organic fertilizers, can solve the problems of uneven distribution and non-renewability of peat resources, damage to the balance of wetland ecosystems, and increased cost of seedling cultivation, etc., to solve resource problems Effects on non-renewable problems, promotion of growth, and reduction of occurrence of soil-borne diseases
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Embodiment 1
[0022] Embodiment 1 The growth-promoting effect of Bacillus amyloliquefaciens
[0023] 1. Activation and fermentation of Bacillus amyloliquefaciens
[0024] Transfer the Bacillus amyloliquefaciens WS-8 preserved on the slant to a 50mL Erlenmeyer flask for seed cultivation
[0025] After cultivating overnight, transfer to the fermentation medium substrate for culture after microscopic examination without bacteria.
[0026] The liquid fermentation medium formula of Bacillus amyloliquefaciens is: starch 0.7%, bean cake powder 1.4%, sucrose 0.2%, yeast extract 0.06%, peptone 0.05%, ammonium sulfate 0.13%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.03% , manganese sulfate 0.01%, sodium chloride 0.02%, calcium carbonate 0.1%, pH7.3-7.5, the number of spores can reach 12 billion / ml after 48 hours of cultivation.
[0027] 2. Growth-promoting effect of Bacillus amyloliquefaciens
[0028] The test set three treatments: blank control (water irrigation), fermentation bro...
Embodiment 2
[0032] Embodiment 2 Preparation of biochar bacterial agent
[0033] The fermented Bacillus amyloliquefaciens fermentation liquid was adsorbed with straw-derived biochar at a mass addition ratio of 15%, 20% and 25%, respectively, and the number of Bacillus amyloliquefaciens in the biochar agent after adsorption was checked regularly. The results were obtained from It can be seen from Table 2 that the adsorption capacity of 20% is the best, and the number of live spores is kept the most. The number of live spores with a storage period of 6 months is relatively stable, and the adsorption capacity is not easy to infect miscellaneous bacteria.
[0034] Table 2 The shelf life of different addition ratios
[0035] .
Embodiment 3
[0036] Example 3 Determination of the volume ratio of organic fertilizer, biochar agent, and straw powder
[0037] Organic fertilizer (biogas residue + chicken manure, wherein the mass ratio of biogas residue: chicken manure = 1:2.5), biochar agent (absorption of Bacillus amyloliquefaciens is 20%) and straw powder are mixed according to different mass parts , it can be seen from Table 3 that the mass ratio of treatment 3, treatment 4 and treatment 5 can effectively improve the germination index and strong seedling index of cucumber, and the difference between the treatments is not significant. Based on the consideration of input cost, organic fertilizer, The optimal mass ratio of biochar agent and straw powder is 7:40:2, which can effectively improve the germination index and strong seedling index of cucumber.
[0038] Table 3 Effects of different components on germination index and strong seedling index
[0039]
[0040] Note: The lowercase letters in the table represent ...
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