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Serratia marcescens, a bacterial agent, and application thereof in degradation of polycyclic aromatic hydrocarbons (PAHs)

A Serratia marcescens, polycyclic aromatic hydrocarbon technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve problems such as endangering biological health, carcinogenicity, teratogenicity, etc. The effect of degradation ability, fast growth rate and strong vitality

Active Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Naphthalene and other polycyclic aromatic hydrocarbons have "three-cause" effects (carcinogenic, teratogenic and mutagenic), which not only directly endanger the health of various organisms, but also endanger human health through bioaccumulation and biomagnification because they are difficult to degrade. seriously damaged the ecological environment

Method used

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  • Serratia marcescens, a bacterial agent, and application thereof in degradation of polycyclic aromatic hydrocarbons (PAHs)
  • Serratia marcescens, a bacterial agent, and application thereof in degradation of polycyclic aromatic hydrocarbons (PAHs)
  • Serratia marcescens, a bacterial agent, and application thereof in degradation of polycyclic aromatic hydrocarbons (PAHs)

Examples

Experimental program
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Effect test

Embodiment 1

[0056] This embodiment is used to illustrate the tolerance and degradation of Serratia marcescens provided by the present invention to naphthalene in LB culture fluid

[0057] The bacterial strain QD-G was inserted into sterilized LB liquid medium containing naphthalene at concentrations of 10 mg / L, 20 mg / L, 50 mg / L, 100 mg / L and 200 mg / L with an inoculum size of 1 volume %. Cultured at 170 rpm, 30±1°C for 24 hours, the results showed that the strain QD-G showed good tolerance to naphthalene, and the strains could grow in different media. The maximum OD value and the degradation rate of naphthalene that the bacteria can achieve are shown in Table 1.

Embodiment 2

[0068] This example is used to illustrate the tolerance and degradation of Serratia marcescens provided by the present invention to naphthalene in the inorganic saline phase

[0069] Inoculate the bacterial strain QD-G with the inoculum amount of 1% by volume into the sterilized MSM inorganic salt medium containing naphthalene at a concentration of 10mg / L, 20mg / L, 50mg / L, 100mg / L and 200mg / L respectively . Cultivate at 170rpm, 30±1°C for 24h. The results showed that the strain QD-G showed good tolerance to naphthalene when naphthalene was used as the sole carbon source. The maximum OD value and the degradation rate of naphthalene that the bacteria can achieve are shown in Table 2.

Embodiment 3

[0080] This example is used to illustrate the tolerance and degradation of Serratia marcescens provided by the present invention to naphthalene in the inorganic saline phase

[0081] The 100ml containing the naphthalene-containing inorganic salt medium (based on 1L inorganic salt medium, containing 10g of NH 4 NO 3 , 1.5g of KH 2 PO 4 , 3g of K2 HPO 4 , 0.008g of MgSO 4 , 0.002 CuSO 4 , 0.002 MnSO 4 , 0.002 FeSO 4 , 0.002 CaCl 2 , pH=7.5). Cultivate for 3 days at 170 rpm, 35±1°C. The degradation rate of naphthalene by strain QD-G was 97%.

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Abstract

The invention relates to the field of microorganism environment repairing and particularly discloses a strain of serratia marcescens. The serratia marcescens is assigned the accession number of CGMCC No.10847. The invention also discloses a bacterial agent comprising the serratia marcescens strain, discloses an application of the bacterial strain and / or the bacterial agent hereinabove in degradation of PAHs, and a method of degrading the PAHs. The serratia marcescens can survive and growth with the PAHs (such as naphthalene) as the only one carbon source under the condition of providing a carbon source, a nitrogen source and inorganic salts only. The serratia marcescens has strong vitality, high growth speed and high biomass, and is very strong in tolerance and degradability on the PAHs.

Description

technical field [0001] The present invention relates to the field of microbial environment remediation, in particular to a new strain of Serratia marcescens, bacterial agents containing the Serratia marcescens, their application in degrading polycyclic aromatic hydrocarbons and a method for degrading polycyclic aromatic hydrocarbons. A method for cycloaromatic hydrocarbons. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are widely distributed in the environment and are a class of pollutants with complex chemical structures, mutagenicity and carcinogenicity. It can be enriched through the transfer of biological chains, causing great harm to the environment and humans. [0003] Naphthalene is a low-molecular-weight polycyclic aromatic hydrocarbon compound composed of two benzene rings, which is widely distributed in natural environments such as air, soil, water and its sediments. Naphthalene and other polycyclic aromatic hydrocarbons have "three-cause"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/43C02F101/32
Inventor 任立伟齐丹林森王培红
Owner 粮华生物科技(北京)有限公司