Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Salvia miltiorrhiza bunge genetic transformation method

A genetic transformation method and technology of Salvia miltiorrhiza, applied in the field of genetic transformation of salvia miltiorrhiza, can solve the problems of time-consuming and laborious and low positive rate of removing false positive seedlings, and achieve the effect of improving the quality of salvia miltiorrhiza

Inactive Publication Date: 2017-03-15
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, some researchers used 10 mg / L Hyg to screen the transgenic plants of Salvia miltiorrhiza in the early stage, and found that the explants of Salvia miltiorrhiza were not easy to brown and die under the screening concentration of 10 mg / L Hyg, and it was easy to obtain regenerated plants of Salvia miltiorrhiza. When the plants are tested, the positive rate is not high, and it takes time and effort to eliminate false positive seedlings in the later stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Salvia miltiorrhiza bunge genetic transformation method
  • Salvia miltiorrhiza bunge genetic transformation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] In order to make the object, technical solution and advantages of the present invention more clear, the method for genetic transformation of Danshen of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0020] For a better understanding of those skilled in the art, some terms are firstly explained as follows.

[0021] Kanamycin, the English name is Kanamycin, abbreviated as Kan.

[0022] Hygromycin, the English name is Hygromycin B, abbreviated as Hyg.

[0023] Cephalosporin, the English name is Cefotaxime, abbreviated as Cef.

[0024] 6-Benzyl Aminopurine, the English name is 6-Benzyl Aminopurine, abbreviated as 6-BA.

[0025] Indole butyric acid, English name is indole-3-Butytric acid, abbreviated as IBA.

[0026] Naphthaleneacetic acid, En...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a salvia miltiorrhiza bunge genetic transformation method which has the advantages of simplicity and convenience in operation, high repeatability and capability of rapidly and effectively acquiring genetically modified salvia miltiorrhiza bunge plants. At present, most salvia miltiorrhiza bunge plant genetic transformation systems adopt Kanamycin (abbreviated as Kan) for screening. However, inventors find that 99-3 salvia miltiorrhiza bunge leaves are sensitive to the Kanamycin in genetic transformation research for 99-3strain salvia miltiorrhiza bunge subjected to sequencing, leaf growth is easily stopped when the Kanamycin is used as a screening antibiotic, and experimental reproducibility is poor. When 30-60mg / L Hygromycin B (abbreviated as Hyg) is used for screening, positive genetically modified plants are obtained, and positive rate is quite high. In conclusion, the salvia miltiorrhiza bunge plant genetic transformation method taking the salvia miltiorrhiza bunge leaves as explants is efficient, reliable and simple and convenient to operate. The method is mediated by agrobacterium tumefaciens, plasmid carrying exogenous genes are transformed into the salvia miltiorrhiza bunge, and the positive genetically modified salvia miltiorrhiza bunge plants are obtained.

Description

technical field [0001] The invention relates to the field of transgenic technology, in particular to a genetic transformation method of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza Bunge (Salvia miltiorrhiza Bunge) is a perennial herb of the genus Salvia in the Labiatae family. It is mainly used as medicine with its dried roots and rhizomes. Salvia miltiorrhiza is bitter in taste, slightly cold in nature, and has the functions of promoting blood circulation and eliminating phlegm, nourishing blood and calming the nerves, reducing swelling and pain, cooling blood and eliminating carbuncle, etc. commonly used medicinal plants. In addition, Salvia miltiorrhiza has few chromosomes, small genome, strong vitality, and short generation cycle. It is a model organism in the study of medicinal plants, and the research on its genome and transcriptome has also made some progress. It is of great significance to reveal the molecular mechanism of the synthesis o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/84A01H5/00
CPCC12N15/8205
Inventor 卢善发邓宇星刘苗苗庞永奇
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products