Method based on genome LoF loci to screening indirectly related kiwi fruit varietal character

A kiwifruit and genome technology, applied in the field of plant genetic resources evaluation and biology, can solve problems such as difficulty in identifying and utilizing species characteristics, and achieve the effects of improving resource evaluation and utilization efficiency, accurate association prediction, high stability and repeatability

Inactive Publication Date: 2017-03-15
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of difficult identification and utilization of kiwifruit species characteristics that can be stably inherited, and to provide a method for screening indirectly related kiwifruit species based on genomic LoF sites. The method starts with genomic LoF...

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  • Method based on genome LoF loci to screening indirectly related kiwi fruit varietal character
  • Method based on genome LoF loci to screening indirectly related kiwi fruit varietal character

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Embodiment 1

[0024] Embodiment 1: The method for screening indirectly associated kiwifruit species based on the genome LoF site of the present embodiment comprises the following steps:

[0025] 1. If figure 1 Step ① Acquisition of species genome sequence. Using public sequence database resources, including the National Center for Biotechnology Information (NCBI for short), the Kiwifruit Information Resource (KIR for short), and the internal database resources of the National Kiwifruit Germplasm Resource Garden (Wuhan), Obtain short genome sequences of kiwifruit species.

[0026] For kiwifruit species that have not released sequence data, the sequence data (genome short fragment sequence) was obtained by low-depth sequencing of the whole genome. According to the genome size of the Chinese kiwifruit reference genome of about 700M, the second-generation genome sequencing of about 5 times lower depth is performed on the kiwifruit species samples to be sequenced, and the total data volume is ...

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Abstract

The invention discloses a method based on genome LoF loci to screening indirectly related kiwi fruit varietal character. The method includes: acquiring a variety genome sequence; comparing with a reference genome of the sequence, discovering and classifying mutation; screening and acquiring LoF mutation loci, and dividing types; performing functional annotation and classification on the LoF mutation loci; screening LoF mutation information unique to varieties, and performing varietal character indirect relation analysis. Compared with previous methods of direct artificial observation on varietal character of kiwi fruit variety and seed breeding experiment detection, the method has higher stability and repeatability, so that relation prediction on the varietal character of the variety is more accurate; by using the method, potential varietal character feature and breedable utilization value of a specific kiwi fruit variety can be quickly acquired and predicted, so that efficiency in resource evaluation and breeding application is improved greatly.

Description

technical field [0001] The invention belongs to the field of plant genetic resource evaluation and biotechnology, and specifically relates to a method for screening indirectly associated kiwifruit species based on genomic LoF sites and its breeding application, that is, the molecular genetic basis for stably inherited kiwifruit species characteristics Analysis and identification, in order to improve the utilization efficiency of kiwifruit resource evaluation and genetic breeding, and promote the rapid cultivation of new varieties. Background technique [0002] my country is rich in wild resources of Actinidia Lindl., and among the 75 taxa of about 54 species in this genus, the natural distribution of most taxa is unique to my country. How to make full use of abundant natural resources, especially wild relatives of cultivated kiwifruit with various shapes, is an important research content of current kiwifruit genetics and breeding work. In the process of evaluating and utili...

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Application Information

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IPC IPC(8): C12Q1/68G06F19/18
CPCC12Q1/6895C12Q2600/156G16B20/00
Inventor 刘义飞李大卫张琼黄宏文
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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