Technique for improving paraffin embedding of cell specimen

A paraffin-embedding and specimen technology, which is applied to the improved technical field of paraffin-embedding of cell specimens, can solve the problems of cell dispersion in the cell paraffin block, damage to the operator's body, long fixation time, etc., and achieves simple operation, clear staining, and fixation. effect of time reduction

Inactive Publication Date: 2017-03-15
吴菡
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  • Application Information

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Problems solved by technology

However, 10% neutral formalin is often used for pre-fixation in the production of cell wax blocks. The fixation time is long, the cells in the cell wax blocks are sc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: The cell sample of the present invention is the cell wax block of specimens such as pleural ascites, bronchoscopic lavage fluid, urine, thoracic and peritoneal washing fluid specimens, lymph nodes, breast and thyroid puncture, and the specific steps for extracting the cell specimen are as follows :

[0066](1) Sedimentary cell specimens: The more fluid specimens to be submitted for inspection, the better, generally between 10 and 500ml, and the specimens should be kept in a cool place for 5 minutes

[0067] (2) Centrifuge cell specimens: Pour off about half of the liquid gently. If it exceeds 100ml, let it stand for 5 minutes and discard half of it. The remaining 50ml of natural sediment specimens should be placed in five 10ml centrifuge tubes at room temperature. Centrifuge at 3000 rpm for 5-10 minutes, blot the supernatant, and use a pipette to suck up the sediment and smear the upper layer of cells

[0068] (3) Fixed cell specimens: pipette the remaini...

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PUM

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Abstract

The invention provides a technique for improving paraffin embedding of a cell specimen. The technique is characterized by comprising the following steps: depositing the cell specimen, fixing, performing centrifugal treatment, extracting the cell specimen, performing conventional fixation, dehydrating, performing transparency treatment, performing paraffin soaking treatment, performing paraffin embedding, slicing, dyeing, and packaging. The technique provided by the invention has the advantages of being convenient and rapid to operate, free of pollution, good in practical effect and very applicable to popularization and use in large hospitals as specimen treatment and dyeing processes can be implemented simultaneously with conventional pathological specimens, and not only is conventional inspection implemented, but also immunohistochemistry and special dyeing can be carried out.

Description

technical field [0001] The invention belongs to the cytological detection method in the fields of clinical pathological diagnosis and scientific research, and specifically relates to an improved technique for paraffin embedding of cell samples. Background technique [0002] Exfoliative cytology examination is one of the effective methods for tumor diagnosis. The small amount of hydrothorax and ascites absorbed during the preparation of ordinary smears cannot represent the status of the entire hydrothorax and ascites. Examine most of the cells in the pleural and ascites in order to observe the morphology of most cells, and the cell wax block is easy to preserve, which greatly improves the sensitivity and specificity of cytopathological diagnosis, and makes up for the limited and insufficient production of conventional cell smears. The inadequacies of multiple stains warrant further research and prospective studies. However, 10% neutral formalin is often used for pre-fixation...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 吴菡
Owner 吴菡
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