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A kind of purification and rejuvenation method of Yulin pesto garlic

A technology for purifying and rejuvenating pesto, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problem of restricting the large-scale production and sustainable and healthy development of Yulin pesto, restricting Yulin pesto from becoming a regional specialty product, It is difficult to accurately determine the content of secondary metabolite components, etc., to achieve the effect of preventing garlic from degrading, inducing chemical defense, and promoting root growth and growth

Active Publication Date: 2018-11-06
GUANGXI YULIN RONGSHANTANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Garlic is a vegetative plant. Since garlic cannot be sexually reproduced, farmers usually grow garlic all year round. The limited natural variation cannot meet the requirements of genetic improvement. It is difficult to accurately determine secondary metabolites by traditional methods. Content and Evaluation of Genetic Variation
Yulin pesto relies on bulbs to reproduce, so that the virus accumulates year by year through garlic seeds and is passed on from generation to generation, resulting in a serious degradation of species, which seriously restricts the large-scale production and sustainable and healthy development of Yulin pesto, and limits the development of Yulin pesto as a regional characteristic product pace
[0006] At present, there are very few studies on the use of garlic stem tips for tissue culture for propagation of Yulin pesto. Although there are reports about tissue culture of other varieties, the effect of tissue culture is poor due to the differences between Yulin pesto varieties and other varieties.
Lack of a culture method and special medium for detoxified tissue culture seedlings of Yulin pesto

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for purifying and rejuvenating Yulin pesto garlic, comprising the following steps:

[0032]a. Select garlic cloves with the typical genetic characteristics of Yulin pesto garlic for sowing. Sowing is 10 days earlier than conventional garlic sowing. When the garlic sprouts protrude from the sheath and the involucre expands, tear the involucre and remove the florets. Yulin pesto garlic grows in the late stage Do not harvest garlic sprouts, delay the harvest period of Yulin pesto garlic for 15 days, and when the garlic sprouts turn yellow and the aerial bulbs mature, harvest aerial bulbs with a diameter greater than 0.5 cm, and finally store the aerial bulbs in a 5- Store at 10°C for later use.

[0033] b. Get the aerial bulb described in step a, rinse it with water after first removing the outer skin, then carry out surface detoxification with alcohol for 1 time, then put it into a mercuric chloride solution with a mass concentration of 0.07% and soak it for 12 m...

Embodiment 2

[0038] A method for purifying and rejuvenating Yulin pesto garlic, comprising the following steps:

[0039] a. Select garlic cloves with the typical genetic characteristics of Yulin pesto garlic for sowing. Sowing is 15 days earlier than conventional garlic sowing. When the garlic sprouts protrude from the sheath and the involucre expands, tear the involucre and remove the florets. Yulin pesto garlic grows in the late stage Do not harvest garlic sprouts, delay the harvest period of Yulin pesto garlic for 20 days, and when the garlic sprouts turn yellow and the aerial bulbs mature, harvest aerial bulbs with a diameter greater than 0.4 cm, and finally store the aerial bulbs in a 6- Store at 10°C for later use.

[0040] b. get the aerial bulbs described in step a, rinse with water after first removing the outer skin, then carry out surface detoxification with alcohol for 3 times, then put into the mercuric chloride solution with a mass concentration of 0.15% to soak for 18min and...

Embodiment 3

[0045] A method for purifying and rejuvenating Yulin pesto garlic, comprising the following steps:

[0046] a. Select garlic cloves with the typical genetic characteristics of Yulin pesto garlic for sowing. Sowing is 11 days earlier than conventional garlic sowing. When the garlic sprouts protrude from the sheath and the involucre expands, tear the involucre and remove the florets. Yulin pesto garlic grows in the late stage Do not harvest garlic sprouts, delay the harvest period of Yulin pesto garlic for 16 days, and when the garlic sprouts turn yellow and the aerial bulbs mature, harvest aerial bulbs with a diameter greater than 0.5 cm, and finally store the aerial bulbs in a 6- Store at 9°C for later use.

[0047] b. Take the aerial bulbs described in step a, remove the skin and rinse with water, then use alcohol to detoxify the surface for 1-3 times, then put it into a mercuric chloride solution with a mass concentration of 0.11% and soak it for 15 minutes before taking it ...

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PUM

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Abstract

The invention relates to the technical field of tissue culture and reproduction, in particular to a purification and rejuvenation method for Yulin fragrant garlic. The purification and rejuvenation method for the Yulin fragrant garlic includes the following steps that a, an aerial bulb is cultivated from the Yulin fragrant garlic; b, the aerial bulb is treated to obtain an aerial bulb tip with 1-3 leaf primordia and with the diameter being 0.1-1.5mm; c, the aerial bulb tip is subjected to inoculated culture so that rootless tissue cultured buds can be obtained; and d, the rootless tissue cultured buds are transferred into an enrichment medium to be further cultivated into garlic detoxication tissue culture seedlings. The purification and rejuvenation method for the Yulin fragrant garlic provided by the invention is suitable for tissue culture reproduction of the Yulin fragrant garlic with the help of the aerial bulb tip. The method has the characteristics that germinating of the aerial bulb tip is early, the growth vigor is fast, and the survival rate is highest; and seedling stems of the tissue culture Yulin fragrant garlic are thick and robust, vitality is tenacious and adaptive capacity is high.

Description

【Technical field】 [0001] The invention relates to the technical field of tissue culture and propagation, in particular to a method for purifying and rejuvenating Yulin pesto garlic. 【Background technique】 [0002] Yulin pesto has been cultivated for more than a thousand years. It is large in size, firm in petals, spicy and juicy, crispy and fragrant in taste, and the color of the stem skin is mostly purple, and also white. Listed as a native product of Guangxi, it has a wide market, high export value and good economic benefits. The production areas are concentrated in Rendong, Renhou, Fumian, Dapingshan and other places. [0003] Garlic has a pungent and warm smell, is rich in protein, fat, sugar, calcium, iron and vitamins A, B, C and other nutrients, and has high medicinal value. The effect of "poisonous gas". Garlic is also a commonly used seasoning. When cooking melons, vegetables, fish, and meat, add a few cloves, which is fragrant and delicious. [0004] Yulin pes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G22/35
CPCA01G22/00A01H4/001A01H4/008
Inventor 韦志厚刘星
Owner GUANGXI YULIN RONGSHANTANG BIOTECH
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