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An antagonistic fungus for controlling bitter gourd wilt and its application

A technology of bacterial strains and microbial strains, applied in the fields of application, fungi, and pest control, can solve the problems of pesticide residues, difficulty in adapting to the requirements of planting models, and high production costs, so as to reduce the use of chemical fertilizers and pesticides, which is beneficial to Pollution-free production and the effect of reducing management expenses

Active Publication Date: 2019-05-10
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, no effective disease-resistant varieties for this disease have been bred. In the past, chemical agents such as methyl bromide can be used to effectively control the occurrence of Fusarium wilt, but it is very polluting to the environment, and it is easy to cause drug resistance of the bacteria, and it will also lead to problems such as pesticide residues. , causing serious damage to the ecosystem, threatening human health and food safety, has been banned by most countries in the world
Although measures such as crop rotation and soil disinfection have a certain effect on the control of Fusarium wilt, their production costs are relatively high, and it is difficult to adapt to the requirements of China's intensive agricultural planting model

Method used

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  • An antagonistic fungus for controlling bitter gourd wilt and its application
  • An antagonistic fungus for controlling bitter gourd wilt and its application
  • An antagonistic fungus for controlling bitter gourd wilt and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Isolation and identification of strains

[0032] 1. Isolation and screening of strains:

[0033] (1) Separation of strains

[0034] The cucumber rhizosphere soil collected from the experimental base of Shandong Agricultural University in Tai'an City, Shandong Province was separated by the soil dilution coating plate method. After the soil sample was gradually diluted, 100 μL was taken to a dilution of 10 3 , 10 4 , 10 5 , 10 6 Spread the diluted solution evenly on the pre-prepared PDA medium plate, place it at 25℃ for constant temperature cultivation, and observe daily after 3 days. When the hyphae grow out, pick the hyphae blocks in time and transfer them to the new PDA plate. Continue to cultivate. When spores are produced, monospore separation and purification are carried out, and the purified strains are numbered and stored on a slope for later use.

[0035] PDA medium formula: 200g potato, 20g glucose, 17g agar, 1000ml tap water.

[0036] (2) Screening of antago...

Embodiment 2

[0060] Example 2: Preparation of strain Q2 biocontrol preparation

[0061] The specific preparation method is as follows:

[0062] 1. Preparation of seed liquid

[0063] The strain of Penicilliumpupurogenum Q2 (CGMCC No. 13165) isolated and preserved in Example 1 stored on the slope of the test tube at 4°C was transferred to a fresh PDA medium plate for activation and cultured at 25°C After 5 days of fresh mycelia, they were transferred to a large number of fresh PDA plates and cultured at 25°C for 10 days. After the spores are produced, scrape them off under aseptic conditions and prepare them with sterile water to a concentration of 1×10 8 The spore suspension of cfu / ml is the seed liquid of strain Q2.

[0064] PDA medium formula: 200g potato, 20g glucose, 20g agar, 1000ml water.

[0065] 2. Preparation of biocontrol agents and antibacterial active substances

[0066] 5mL Q2 seed liquid was inoculated into the sterilized fermentation culture material. The formula of the fermentation ...

Embodiment 3

[0067] Example 3: Application of Penicillium violaceum-producing strain Q2 in the prevention and control of bitter gourd wilt

[0068] 1. Test method:

[0069] The experiment has two treatments, namely:

[0070] (1) Treatment group (Q2): The bitter gourd seeds were placed in a spore suspension for 17 hours in a shaker at 33°C and 200r / min, and the surface moisture of the seeds was wiped off, and then placed in sterilized gauze at 33°C. Sterilize at constant temperature for 48-64h, and moisturize with the spore suspension of strain Q2.

[0071] (2) Control group (CK): The treatment methods and conditions of bitter gourd seeds are the same as those in the treatment group. The spore suspension is replaced with sterile water for soaking and moisturizing.

[0072] After accelerating germination, the white seeds were sown in a seedling tray filled with sterile soil, and the seedlings were grown in a light incubator (16h light, 28℃, 8h dark, 22℃), and regular moisture management.

[0073] The ...

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Abstract

The present invention discloses a Penicillium purpureogenum strain Q2, which has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, referred to as CGMCC, on October 25, 2016. The address is: 1 Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, strain preservation number is CGMCC NO.13165. The present invention is the Penicillium purpurea Q2 bacterial strain isolated from cucumber rhizosphere soil for the first time. The bacterial strain has a strong antagonistic effect on the plant soil-borne disease bitter gourd wilt, and may be biological control of bitter gourd fusarium wilt and other horticultural crop soil-borne diseases. Provide new bio-control bacteria, increase the types of bio-control agents, and solve the phenomenon of single bio-control agents in the market.

Description

Technical field [0001] The invention relates to an antagonistic fungus for preventing and treating bitter gourd wilt and its application, in particular to a strain of Penicillium purpureus which has a significant control effect on bitter gourd wilt and its application, and belongs to the field of biotechnology. Background technique [0002] Bitter gourd wilt (bitter gourd wilt) is a soil-borne disease caused by Fusarium oxysporumf.sp.momordicae that infects bitter gourd. It is widely distributed in various bitter gourd producing areas in the world. It is in the whole growth period of bitter gourd. It can happen. When the disease is epidemic, a large number of ayahuasca can appear in the melon field, reducing the yield by more than 30%, which seriously affects the yield and quality of bitter gourd. [0003] At present, there is no effective disease-resistant variety against the disease. In the past, the use of methyl bromide and other chemicals can effectively control the occurrence...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80Y02A40/10
Inventor 高克祥田叶韩刘晓光王永阳何邦令
Owner SHANDONG AGRICULTURAL UNIVERSITY
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