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Method for converting avermectin dreg into lactococcus lactis, and culture medium thereof

A technology of Lactococcus lactis and culture medium, which is applied in the field of reuse of antibiotic fermentation residue, can solve problems such as contamination of Avermectin residue, reduce production costs, improve economic benefits, realize recycling and high-value transformation Effect

Active Publication Date: 2017-03-22
INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects in the prior art, the object of the present invention is to provide a method and culture medium for converting Avermectin slag into lactococcus, so as to solve the pollution problem of Avermectin slag to the environment and realize the At the same time, it provides a source of raw materials for the production of lactococcus, realizes the recycling and high-value transformation of Avermectin residue, reduces production costs, and improves economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Dry the Avermectin residue at 105°C and then pulverize it to obtain the dry powder of Avermectin residue, then pass through a 60-mesh sieve to collect the dry powder of Avermectin residue with a particle size less than or equal to 0.250mm; weigh 9g of the dry powder of Avermectin residue , join KH 2 PO 4 , NaCl and MgSO 4 ·7H 2 O, add distilled water to 50mL again, make Avermella slag be 180g / L by dry weight, KH 2 PO 4 Final concentration is 15g / L, NaCl final concentration is 1.0g / L, MgSO 4 ·7H 2The final concentration of O is 0.10g / L, mix well, adjust the pH of the obtained medium to 6.8, and cool to 30°C after autoclaving at 121°C for 20 minutes to obtain the sterilized medium. Lactococcus lactis subsp.lactis YF11 cultivated to the mid-logarithmic phase was inoculated into the sterilized medium with an inoculum amount of 3% (the volume ratio of the inoculated strain amount to the sterilized medium) , after culturing at a temperature of 28°C and a stirring speed...

Embodiment 2

[0031] Dry the Avermectin residue at 105°C and then powder it to obtain the dry powder of Avermectin residue, then pass through a 60-mesh sieve to collect the dry powder of Avermectin residue with a particle size less than or equal to 0.250mm; weigh 12g of the dry powder of Avermectin residue , join KH 2 PO 4 , NaCl and MgSO 4 ·7H 2 O, add distilled water to 50mL again, make Avermella slag be 240g / L by dry weight, KH 2 PO 4 Final concentration is 20g / L, NaCl final concentration is 1.5g / L, MgSO 4 ·7H 2 The final concentration of O is 0.15g / L, mix well, adjust the pH of the obtained medium to 7.0, and cool to 30°C after autoclaving at 121°C for 20 minutes to obtain the sterilized medium. Lactococcus lactis subsp.lactis YF11 cultivated to the mid-logarithmic phase was inoculated into the sterilized medium at an inoculation amount of 5% (the volume ratio of the inoculated strain amount to the sterilized medium) , after culturing at a temperature of 30°C and a stirring speed...

Embodiment 3

[0033] Dry the Avermectin residue at 105°C and then pulverize it to obtain the dry powder of Avermectin residue, then pass through a 60-mesh sieve to collect the dry powder of Avermectin residue with a particle size less than or equal to 0.250mm; weigh 18g of the dry powder of Avermectin residue , join KH 2 PO 4 , NaCl and MgSO 4 ·7H 2 O, add distilled water to 50mL again, make Avermella slag be 360g / L by dry weight, KH 2 PO 4 Final concentration is 25g / L, NaCl final concentration is 2.0g / L, MgSO 4 ·7H 2 The final concentration of O is 0.20g / L, mix well, adjust the pH of the obtained medium to 7.2, and cool to 32°C after autoclaving at 121°C for 20 minutes to obtain the sterilized medium. Lactococcus lactis subsp.lactis YF11 cultivated to the mid-logarithmic phase was inoculated into the sterilized medium with an inoculation amount of 8% (the volume ratio of the inoculated strain amount to the sterilized medium) , after culturing at a temperature of 30°C and a stirring ...

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Abstract

The invention relates to a method for converting avermectin dregs into lactococcus lactis, and a culture medium thereof. The culture medium comprises the avermectin dregs. According to the method for utilizing the avermectin dregs for producing the lactococcus lactis provided by the invention, the problem that the environment is polluted by the avermectin dregs can be solved, the recycling of the avermectin dregs is realized, meanwhile, a raw material source is provided for the production of the lactococcus lactis, the recycling and the high-valued conversion of the avermectin dregs are realized, the production cost is reduced, and the economic benefit is improved.

Description

technical field [0001] The invention relates to the technical field of antibiotic fermentation residue reuse, in particular to a method for converting avermectin residue into lactococcus and a culture medium thereof. Background technique [0002] Abamectin is a sixteen-membered macrocyclic lactone compound, which is a biopesticide that kills insects and miticides. In 1975, it was isolated from Streptomyces avermitilis by Satoshi Omura of Kitasato Institute in Japan. Unlike most pesticides, the mechanism of action of abamectin is to interfere with the neurophysiological activities of pests, stimulate the release of GABA to inhibit the nerve conduction of arthropods, but is harmless to vertebrates. Because of its high efficiency, broad spectrum, safety, and less residue, it has been widely used in agriculture, animal husbandry, and medicine. [0003] Abamectin residue is the substance left after extracting avermectin during the production process of avermectin. Its main comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/46
CPCC12P21/02
Inventor 张小利吕烨王硕季祥李雅丽张楠赵鑫茹
Owner INNER MONGOLIA UNIV OF SCI & TECH
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