Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting ethanol in live bacteria type lactic acid bacteria beverage

A technology of lactic acid bacteria beverage and ethanol, which is applied in the direction of measuring devices, material separation, and analysis of materials, etc., can solve the problems of ethanol that need to be improved, and achieve the effects of fast operation, high precision, and strong accuracy

Active Publication Date: 2019-04-26
蒙牛乳业(马鞍山)有限公司 +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current method for detecting ethanol in live lactic acid bacteria beverages still needs to be improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting ethanol in live bacteria type lactic acid bacteria beverage
  • Method for detecting ethanol in live bacteria type lactic acid bacteria beverage
  • Method for detecting ethanol in live bacteria type lactic acid bacteria beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] In this embodiment, the ethanol in the live bacteria type lactic acid bacteria beverage is detected through the following steps:

[0056] The first step is to draw 5mL live lactic acid bacteria beverage test sample into a 25mL volumetric flask, dilute to the mark with acetonitrile, mix well, centrifuge at 5000rpm for 5 minutes, collect the supernatant, and pass through a 0.22μm organic filter membrane. Obtain the solution to be tested.

[0057] In the second step, the liquid to be tested is detected by gas chromatography:

[0058] (1) Determine the detection limit of gas chromatography

[0059] The ethanol can be qualitatively determined by a 3-fold signal-to-noise ratio (S / N=3), and the ethanol can be quantified by a 10-fold signal-to-noise ratio (S / N=10). When the content is 2mg / 100mL, the peak height signal is 2146.8, the noise is 70, and the signal-to-noise ratio at this time is 30.7. Based on 10 times the signal-to-noise ratio, the quantitative detection limit of...

Embodiment 2

[0069] Detect ethanol in the live bacteria type lactic acid bacteria beverage according to the method of Example 1, the difference is that in the first step,

[0070] Draw 5mL of live bacteria type lactic acid bacteria beverage to be tested into a 10mL volumetric flask;

[0071] Centrifugation is carried out at a speed of 3000r / min for 10 minutes.

Embodiment 3

[0073] Detect ethanol in the live bacteria type lactic acid bacteria beverage according to the method of Example 1, the difference is that in the first step,

[0074] Draw 5mL of live bacteria type lactic acid bacteria beverage to be tested into a 50mL volumetric flask;

[0075] Centrifugation is carried out at a speed of 8000r / min for 3 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for detecting ethanol in a living lactobacillus beverage. The method comprises the following steps: (1) mixing a living lactobacillus beverage with acetonitrile, subjecting the mixture to a centrifugal treatment, and collecting the supernate; and (2) subjecting the supernate to chromatographic detection, and determining the ethanol content of the living lactobacillus beverage based on the detection result. The provided method at least has one advantage of convenient operation, rapidness, high precision, and high accuracy.

Description

technical field [0001] The invention relates to the field of food. Specifically, the present invention relates to a method for detecting ethanol in live lactic acid bacteria beverages. Background technique [0002] Live lactic acid bacteria beverages are easily contaminated by yeast during the production process, resulting in swelling of the product and affecting product quality. Utilizing the metabolic characteristics of ethanol produced by yeast fermentation, by detecting the content of ethanol, it can be judged whether the product is contaminated by yeast. [0003] However, the current methods for detecting ethanol in live lactic acid bacteria beverages still need to be improved. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, an object of the present invention is to provide a method for detecting ethanol in live lactic acid bacteria beverages, which is simple, fast, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 韩磊李光辉吴小慧王伟金珠王丹慧李梅
Owner 蒙牛乳业(马鞍山)有限公司