Sidestream dark field imaging technique-based human body microvessel three-dimensional ultrastructure imaging system

Abstract

The present invention relates to a sidestream dark field imaging technique-based human body microvessel three-dimensional ultrastructure imaging system. The system at least comprises a light source module, an aperture module, a light splitting unit, a dark field objective lens, an imaging unit and an image display unit, wherein the light source module is used for emitting linearly-transmitted columnar light, the aperture module is used for making light irradiating the surface of a measured object become irradiation position-constant and irradiation angle-varied circularly polarized light, the dark field objective lens is used for irradiating the light onto the surface of the measured object in a concentrated manner, the imaging unit is used for imaging, and the image display unit is used for displaying an image. The aperture module is rotated regularly, so that incident light entering the dark field objective lens can be annular incident light; based on a special light system inside the dark field objective lens, emergent light irradiates the surface of the measured object in an inverse-frustum-shaped manner; and therefore, a microvascular ultrastructure in measured skin at the same position can be irradiated by light with different angles, and the principle of photometric three-dimensional imaging can be complied, and three-dimensional imaging can be achieved, and the microvessel density and microvascular ultrastructure of a longitudinal section can be accurately digitized and quantified.

Description

technical field [0001] The invention relates to a medical device for medical diagnosis using optical imaging technology, in particular to a three-dimensional ultramicrostructure imaging system of human microvessels based on lateral flow dark field imaging technology. Background technique [0002] Microcirculation refers to the place where blood and tissue cells exchange substances between arterioles and venules. The integrity of the function, shape and metabolism of the microcirculation is an indispensable condition for maintaining the normal function of human organs. Through the study of microcirculation, it is convenient to further understand the special functions of various organs of the human body, and to recognize the pathogenesis of diseases, which is beneficial to disease prevention, diagnosis and treatment. A variety of disease states, including diabetes, hypertension and coronary heart disease, will cause pathological changes in microcirculation, including changes ...

AI Technical Summary

Problems solved by technology

[0006] However, traditional lateral flow dark-field imaging techniques can only achieve two-dimensional imaging

Although the analysis of two-dimensional imaging can quantify and digitize the ultrastructural flow velocity of microvessels, the diameter of microvessels, and the microvessel density in cross-section, because two-dimensional imaging cannot obtain depth information, the microvessel density and shape of microvessels in longitudinal section For analysis, 2D imaging does not suffice

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