A kind of pelcl/polycaprolactone-g-polyethylene glycol-redv electrospun fiber membrane and preparation method

A polyethylene glycol and caprolactone technology, applied in the field of biomedical materials, can solve the problems of increasing the cost of raw materials and reducing the activity of biologically active substances, and achieve good fiber morphology, good biological activity, and promote the adhesion of vascular endothelial cells and growth effects

Inactive Publication Date: 2019-06-25
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, by surface chemical modification, coupling small peptides on the surface of electrospun fiber membranes loaded with bioactive substances, such as acid, alkali or ammonolysis treatment, may reduce the activity of bioactive substances, thereby increasing the cost of raw materials

Method used

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  • A kind of pelcl/polycaprolactone-g-polyethylene glycol-redv electrospun fiber membrane and preparation method
  • A kind of pelcl/polycaprolactone-g-polyethylene glycol-redv electrospun fiber membrane and preparation method
  • A kind of pelcl/polycaprolactone-g-polyethylene glycol-redv electrospun fiber membrane and preparation method

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Experimental program
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preparation example Construction

[0035] The preparation method of polyε-caprolactone-g-polyethylene glycol-REDV is characterized in that comprising steps:

[0036] (1) Amination modification of polyε-caprolactone: prepare polyε-caprolactone into 0.3-0.5g / mL anhydrous dichloromethane solution, and use N,N'-carbonyldiimidazole (CDI) to activate Hydroxyl group, add CDI according to the molar ratio of [single-end hydroxyl polyε-caprolactone] / [CDI] 1:1 (or [double-end hydroxyl polyε-caprolactone] / [CDI] 1:2) , at N 2 Stir and react at room temperature for 24 hours, then add excess ethylenediamine, stir and react at room temperature for 48 to 72 hours, precipitate the product with excess methanol, and dry it in vacuum to obtain the amino-modified product of polyε-caprolactone.

[0037] (2) Preparation of poly-caprolactone-g-polyethylene glycol: prepare the amino-modified poly-caprolactone into a dichloromethane solution with a solubility of 200-300 mg / mL, according to the polyε-caprolactone The molar ratio of the ...

Embodiment 1

[0043] In a three-neck flask equipped with magnetic stirring, 3 g of polyε-caprolactone ( double-terminated hydroxyl) was dissolved in 10mL of anhydrous dichloromethane, according to the molar ratio [double-terminated polyε-caprolactone] / [CDI] was 1:2 to add CDI65.0mg, in N 2 The reaction was stirred at room temperature for 24 hours, then 2.0 mL of ethylenediamine was added, and the reaction was stirred at room temperature for 48 hours. The product was precipitated with excess methanol and dried in vacuum for 24 hours to obtain an amino-modified polyε-caprolactone.

[0044] Dissolve 78 mg of the amino-modified polyε-caprolactone in 3 mL of dichloromethane solution, and the molar ratio of the amino group in the amino-modified polyε-caprolactone to the NHS group in the double-ended PEG is 1 : 1 Add double-ended PEG, maleimide-polyethylene glycol-succinimide acetate (MAL-PEG-NHS, ) 208 mg, stirred at room temperature for 6 h, and the product was precipitated with ethanol to ob...

Embodiment 2

[0047] In a three-neck flask equipped with magnetic stirring, 5 g of polyε-caprolactone ( double-terminal hydroxyl) was dissolved in 10mL of anhydrous dichloromethane, according to the molar ratio [double-terminal hydroxyl polyε-caprolactone] / [CDI] is 1:2, add CDI 32.4mg, in N 2 The reaction was stirred at room temperature for 24 hours, then 2.0 mL of ethylenediamine was added, and the reaction was stirred at room temperature for 60 hours. The product was precipitated with excess methanol and dried in vacuum for 24 hours to obtain an amino-modified polyε-caprolactone.

[0048] Dissolve 600 mg of the amino-modified polyε-caprolactone in 3 mL of dichloromethane solution, and the molar ratio of the amino group in the amino-modified polyε-caprolactone to the NHS group in the double-ended PEG is 1 :1 Add double-ended PEG, maleimide-polyethylene glycol-succinimide acetate (MAL-PEG-NHS, ) 120 mg, stirred at room temperature for 10 h, and the product was precipitated with ethanol t...

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Abstract

The invention relates to a polyethylene glycol-b-poly(L-lactide-co-epsilon-caprolactone) / poly epsilon-caprolactone-g-polyethylene glycol-REDV blend ultrafine electrospun fiber membrane and a preparation method. The method comprises the steps that polyethylene glycol-b-poly(L-lactide-co-epsilon-caprolactone) and poly epsilon-caprolactone-g-polyethylene glycol-REDV are together dissolved into a mixed solvent of chloroform and N,N-dimethylformamide according to the volume ratio 4:(1-8):1; electrospinning is conducted on the solution on the conditions that the flow is 0.02-0.10 mL / h, the voltage is 12-20 kV, and the receiving distance is 15-20 cm, and the electrospun ultrafine fiber membrane with the thickness being 50-200 micrometers. The ultrafine fiber membrane surface contains REDV small peptide and is good in promotion of vascular endothelial cell adhesion and growth effect; the membrane is used for encapsulating bioactive substances such as nucleic acid, protein and medicine, has the function of controlling released bioactive substances and is used in the field of biomedical materials.

Description

technical field [0001] The invention relates to a polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) (PELCL) / polycaprolactone-g-polyethylene glycol-REDV electrospun ultrafine A fiber membrane and a preparation method thereof belong to the field of biomedical materials. Background technique [0002] Using electrospinning technology to load bioactive substances such as gene proteins in tissue engineering scaffolds can regulate cell adhesion, proliferation and migration, and is beneficial to tissue repair and regeneration. Biodegradable polylactide copolymer polyethylene glycol-b-poly(L-lactide-co-ε-caprolactone) (PELCL), polyε-caprolactone (polycaprolactone, PCL), etc. Good biocompatibility and excellent mechanical properties. Electrospinning technology was used to prepare ultrafine fiber membranes, which simulated the structure of extracellular matrix and could be used as scaffold materials for tissue reconstruction. Arginine-glutamic acid-aspartic acid-valine (Arg-Gl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/54A61L27/50D04H1/728D04H1/4382C08G81/00
CPCA61L27/18A61L27/507A61L27/54A61L2300/214A61L2300/412A61L2400/18C08G81/00D04H1/4382D04H1/728C08L87/005
Inventor 袁晓燕周芳任丽霞赵蕴慧崔策文美玲
Owner TIANJIN UNIV
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