Chimeric protein pAgoE, construction method and applications thereof, chimeric protein pAgoE using guide, and construction method and applications thereof

A chimeric protein and construction method technology, applied in chemical instruments and methods, biochemical equipment and methods, hybrid peptides, etc., can solve problems such as unclear methods and reproducibility crises
CN106589134AActive Publication Date: 2017-04-26仪宏
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
仪宏
Publication Date
2017-04-26

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Abstract

The invention relates to biological technology, specifically relates to a chimeric protein pAgoE, a construction method and applications thereof, and at the same time, relates to a chimeric protein pAgoE using a guide, a construction method and applications thereof, and a chimeric protein, which is based on the chimeric protein pAgoE and has a genome targeting editing function. The chimeric protein pAgoE contains a pAgo structural domain with a genome targeting positioning function and an effect structural domain E, which is connected to the pAgo structural domain and has a genome cutting or modifying activity. The pAgo structural domain with a genome targeting positioning function and the effect structural domain E with a genome cutting or modifying activity are fused to establish the chimeric protein pAgoE; the chimeric protein pAgoE maintains the original targeting binding performance of the genome (DNA) having a targeting positioning function in the pAgo structural domain and the genome catalytic or modifying performance of the effect structural domain E at the same time, and an sufficient space is provided for the structural domain E to fully exert the effect.
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Description

technical field

[0001] The present invention relates to biotechnology, specifically, the present invention relates to a kind of chimeric protein pAgoE and its construction method and application, meanwhile, the present invention also relates to a kind of chimeric protein pAgoE using guide and its construction method and application, and based on Chimeric protein pAgoE is a chimeric protein with genome-targeted editing function.

[0002] technical background

[0003] Targeted genome editing technology and targeted genome modification technology are a general term for a type of high-efficiency genome rewriting technology. Its main advantages over gene recombination technology in the general sense are: high genome targeting accuracy, high gene editing efficiency, and a wide range of applications , Mammalian cells can be manipulated. Targeted genome editing technology and targeted genome modification technology can carry out functional research and genetic modification of the ge...

Claims

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