Mutation screening method of Mucor sufu strain, fermented bean curd, and production method of fermented bean curd
A technology for mutagenesis screening and production methods, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as affecting the quality of fermented bean curd and the taste of fermented bean curd becoming worse.
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[0088] A method for mutagenesis screening of Sufu Mucor species, comprising the steps of:
[0089] (1), the starting strain is Mucorus tallum. Optionally, the characteristics of the original bacteria are determined, such as spore growth at different temperatures, protease activity, growth rate and other characteristics of the original bacteria.
[0090] (2), use potato culture medium, purify mucormyces tallow strains, and cultivate for 48 hours, wait for the spores to plump and set aside.
[0091] (3), adopting 0.01% sodium lauryl sulfate solution to make the mucormycetes species into a spore concentration of (0.1~9.99)×10 6 spore bacteria suspension per milliliter; specifically: 15 milliliters of spore bacteria suspension was prepared with 0.01% sodium lauryl sulfate solution, put into a triangular flask, and glass beads were added, and the spores were broken up by shaking. Filter with absorbent cotton to house 30 milliliters of 0.01% sodium lauryl sulfate solution, so that...
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