A modification method of natural anticancer peptide

Abstract

The invention relates to the biotechnology field, specifically relates to an improvement method of natural anticancer peptide. The method comprises the design improvements at two stages by use of natural polypeptide as a template; in the design improvement at the first stage, a series of polypeptide derivatives are obtained by changing the percentage of polypeptide net charge and the hydrophobic residue in the polypeptide; in the design improvement at the second stage, the variety and the number of the screened polypeptide amino acid residue are unchanged, only the primary amino acid sequence is changed, so that the alpha-helix in the high-level structure is located at different positions or disappeared to obtain the series of polypeptide again. The anticancer peptide prepared by use of the method disclosed by the invention is not only high in anticancer property, but also small in toxicity and safer to the human body.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for transforming natural anticancer peptides. Background technique [0002] Anticancer peptides (ACPs) are a class of cationic amphipathic small peptides with no more than 50 amino acid residues and contain basic and hydrophobic residues (Diana Gaspar et al., 2013). Since the surface of microbial cell membranes and tumor cell membranes are rich in negatively charged glycolipids and (or) glycoproteins, many antimicrobial peptides (Antimicrobalpeptides, AMPs) often have dual functions of antibacterial and antitumor (Diana Gaspar et al., 2013 ; David W. Hoskin et al., 2008). Both ACPs and AMPs can be located on the cell membrane through the electrostatic interaction between their positive charges and the negatively charged molecules on the surface of the tumor cell membrane or microbial cell membrane, and then gather on the membrane and be embedded in the cell membrane or pene...

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Problems solved by technology

Although the resistance of tumor cells to ACPs has not been reported, some pathogens, such as Streptococcus, Staphylococci and Enterococcus faecalis, have been reported to be resistant to AMPs (Rashmi Kumariya et al., 2015; Hwang-Soo Joo et al., 2015; Christopher N. LaRock et al., 2015)

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