idl4 mature polypeptide plant aging accelerator, preparation method and application

A mature polypeptide, IDL4 technology, applied in the fields of botanical equipment and methods, plant growth regulators, plant growth regulators, etc., can solve the problem of no plant senescence, and achieve convenient operation, simple preparation and use methods, and convenient acquisition. Effect

Active Publication Date: 2019-03-26
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few studies on the application of mature polypeptides in plants, ...

Method used

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  • idl4 mature polypeptide plant aging accelerator, preparation method and application
  • idl4 mature polypeptide plant aging accelerator, preparation method and application
  • idl4 mature polypeptide plant aging accelerator, preparation method and application

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preparation example Construction

[0027] Another embodiment of the present invention provides a method for preparing an IDL4 mature polypeptide plant senescence promoter. Specifically, the method includes the following steps:

[0028] S1 weighs the IDL4 mature polypeptide powder, dissolves it in water or dimethyl sulfoxide solvent, and prepares the IDL4 mature polypeptide mother liquor with a concentration of 8-10 mmol / L for later use.

[0029] In this step, since the dimethyl sulfoxide has better solubility for the polypeptide, it can better promote the dissolution of the polypeptide and better promote the polypeptide to provide corresponding functions. Since IDL4 mature peptides have high requirements for storage temperature (not easy to store at room temperature for more than 24 hours), the IDL4 mature peptide mother liquor is ready for use. If not used for more than one month, the mother liquor should be stored at -70℃; if used within one month , The mother liquor is stored at -20℃.

[0030] S2 prepare 1 / 2MS liq...

Embodiment 1

[0039] IDL4 mature polypeptide plant senescence promoter, including: 10μmol / L IDL4 mature polypeptide, 3mmol / L 2-(N-morpholine)ethanesulfonic acid solution; the 2-(N-morpholine)ethanesulfonic acid solution is 2- The (N-morpholine) ethanesulfonic acid solution monohydrate was dissolved in 1 / 2 MS liquid medium or MS liquid medium, and the pH of the 2-(N-morpholine) ethanesulfonic acid solution was 5.8.

[0040] The preparation method is as follows:

[0041] (1) Weigh the IDL4 mature polypeptide powder, dissolve it in dimethyl sulfoxide solvent, and prepare the IDL4 mature polypeptide mother solution with a concentration of 10 mmol / L for later use;

[0042] (2) Prepare 1 / 2MS liquid medium, weigh out a quantitative amount of 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above 1 / 2MS liquid medium, prepare a 3mmol / L solution, adjust The pH value of the solution is 5.8. Mix and dissolve thoroughly to obtain 2-(N-morpholine)ethanesulfonic acid solution;

[0043] (3) Add 50 μ...

Embodiment 2

[0045] IDL4 mature polypeptide plant senescence promoter, including: 8μmol / L IDL4 mature polypeptide, 2.5mmol / L 2-(N-morpholine)ethanesulfonic acid solution; the 2-(N-morpholine)ethanesulfonic acid solution is 2 -(N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of the 2-(N-morpholine)ethanesulfonic acid solution was 5.9.

[0046] The preparation method is as follows:

[0047] (1) Weigh the IDL4 mature polypeptide powder, dissolve it in dimethyl sulfoxide solvent, and prepare the IDL4 mature polypeptide mother solution with a concentration of 8 mmol / L for later use;

[0048] (2) Prepare MS liquid medium, weigh out a quantitative amount of 2-(N-morpholine)ethanesulfonic acid monohydrate and add it to the above MS liquid medium to prepare a 2.5mmol / L solution, and adjust the pH of the solution For 5.9, mix and dissolve well to obtain 2-(N-morpholine)ethanesulfonic acid solution;

[0049] (3) Add 100 μL of the IDL4 mature polypeptide mo...

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Abstract

The invention provides an IDL4 mature polypeptide plant senescence accelerator, a preparation method and application thereof and belongs to the field of plant senescence accelerators. The plant senescence accelerator can accelerate leaf senescence of plants to accelerate senescence of the plants and has no other extra bad phenotypes. A functional ingredient of the plant senescence accelerator is IDL4 mature polypeptide, and an amino acid sequence of the ILD4 mature polypeptide is shown as Pro-Val-Pro-Ala-Ser-Ala-Pro-Ser-Arg-Lys-His-Asn. The IDL4 mature polypeptide plant senescence accelerator comprises the IDL4 mature polypeptide and 2-(N-morpholine) ethanesulfonic solution which is formed by dissolving 2-(N-morpholine) ethanesulfonic monohydrate in 1/2 MS liquid culture medium or MS liquid culture medium. The IDL4 mature polypeptide plant senescence accelerator can be used for accelerating plant senescence.

Description

Technical field [0001] The invention relates to the field of plant senescence promoters, in particular to an IDL4 mature polypeptide plant senescence promoter, and a preparation method and application thereof. Background technique [0002] Leaf senescence is an important factor affecting agronomic traits such as crop yield and quality. Take tobacco as an example. Tobacco is an important economic crop. The leaves are the harvest organs. As the tobacco enters the later stage of growth, the leaves enter the senescence stage. The upper, middle and lower leaves of tobacco have different maturity periods. Generally, different parts and different periods are used in production. The completion of the harvesting strategy has delayed the harvesting time; on the other hand, environmental factors such as continuous rainy days near the harvesting period may cause the tobacco leaves to "return green" and affect the harvest. Therefore, the use of senescence promoters can promote the senescence...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01P21/00A01G7/06
CPCA01G7/06A01N37/46
Inventor 郭永峰张增林侯智慧李伟高晓明徐萌萌
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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