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A method for efficiently preparing low molecular weight pectin

A low-molecular-weight, pectin technology, applied in the field of high-efficiency preparation of low-molecular-weight pectin, can solve the problems of inefficiency, time-consuming, unfriendly environment, and difficult to control, and achieve the effects of promoting application, shortening time, and improving preparation efficiency.

Active Publication Date: 2019-03-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently, the methods used to prepare low-molecular-weight pectin mainly include chemical and enzymatic methods. The difficulties in their preparation are inefficient and time-consuming, difficult to control, and unfriendly to the environment. Therefore, it is beneficial to invent an environmentally friendly and efficient preparation method The complex molecular structure and functional effects of pectin are conducive to making full use of pectin in waste resources such as citrus and apple pomace in my country and protecting the environment. It is of great significance to promote the application of pectin polysaccharides in the food industry and pharmaceutical fields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of pectin solution (5g / L)

[0026] Weigh 0.3g of pectin, add 60ml of deionized water, stir to dissolve it, and obtain a 5g / L pectin solution, which is ready-to-use.

[0027] 2 Dielectric Barrier Discharge Low Temperature Plasma Treatment

[0028] Experimental group: Take 2 mL of the above-mentioned pectin solution in a reactor, and conduct dielectric barrier discharge low-temperature plasma treatment on the solution. The experimental conditions are set as follows: air is used as the discharge medium, the air gap distance is set to 2.5mm, the processing voltage is 50V, and the processing time is 50s.

[0029] Control group: 2 mL of the above pectin solution was not treated with low-temperature plasma, but placed at room temperature for 50 seconds.

[0030] 3. Molecular weight determination of pectin polysaccharide

[0031] (1) Dialysis

[0032] The solution treated with dielectric barrier discharge low-temperature plasma and the pectin solution of the ...

Embodiment 2

[0037] 1. Preparation of pectin solution (5g / L)

[0038] Same as the method of one in Example 1.

[0039] 2 Dielectric Barrier Discharge Low Temperature Plasma Treatment

[0040] It is basically the same as the method of the second in the embodiment 1, only the processing voltage is changed to 75V.

[0041] 3. Molecular weight determination of pectin polysaccharide

[0042] Same as the third method in Example 1.

[0043] The results are as follows: the molecular weight of pectin in the control group was 490kDa, and the molecular weight of pectin decreased to 120kDa after dielectric barrier discharge low-temperature plasma treatment. It can be seen that the method of the present invention is more effective in preparing low-molecular-weight pectin under the condition of 75V for 50s.

[0044] Compared with Example 1, this example has a better effect of preparing low molecular weight pectin.

Embodiment 3

[0046] Experimental group condition: treatment voltage is 50V, and treatment time is 90s, and air gap spacing is 2.5mm, and other is with embodiment 1, and the molecular weight of control group pectin is 490kDa, and the molecular weight of pectin after low dielectric barrier discharge temperature plasma treatment reduces to 67kDa. Compared with Example 1, the processing time increases, and the degradation effect is enhanced.

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PUM

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Abstract

The invention discloses a method for preparing low molecular weight pectin. The method comprises the following steps: firstly, dissolving the pectin into water to obtain a pectin solution; carrying out dielectric barrier discharge low temperature plasma treatment on the pectin solution under the treatment conditions that air is used as a discharge medium, the air-gap separation is set to be 2 to 4mm, the treatment voltage is 50 to 75V and the treatment time is 50 to 90s; secondly, dialyzing solution subjected to the dielectric barrier discharge low temperature plasma treatment for 20 to 25 hours; after the dialysis is finished, passing through a water film with the size of 0.3 to 0.5(mu)m to obtain a finished product. According to the method disclosed by the invention, the pectin is treated by using the dielectric barrier discharge low temperature plasma treatment, so that the time of preparing the low molecular weight pectin is greatly shortened.

Description

technical field [0001] The invention relates to the technical field of food engineering, in particular to a method for efficiently preparing low molecular weight pectin. Background technique [0002] Pectin is an acidic heteropolysaccharide existing in the primary wall and middle layer of plant cells, mainly composed of D-galacturonic acid linked by ɑ-1,4-glycosidic bonds. It has biological activities such as lowering blood sugar and lowering blood lipids. It can be degraded and prepared into low molecular weight pectin, which can significantly improve its biological activity and show some new biological activities, such as being used as a plant immune activation factor to induce plant resistance reactions; As a bifidobacterium proliferation factor, improve the structure of the flora; inhibit the accumulation of fat in mouse liver cells, etc., and has broad development and application prospects in the fields of food, medicine, and agriculture. [0003] Currently, the method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/06
CPCC08B37/0045
Inventor 刘东红丁甜李娇张颂叶兴乾陈健初胡亚芹陈士国
Owner ZHEJIANG UNIV