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Whole blood genomic DNA high-flux plate type extracting kit and extracting method

A genome and high-throughput technology, applied in the biological field, can solve problems such as the inability to achieve high-throughput extraction solutions, the inability to achieve high-throughput, automated operations, and complex extraction processes, so as to avoid the risk of burns, low cost, and Effect of Sensitivity Improvement

Inactive Publication Date: 2017-05-10
LUOYANG G N T BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

In summary, this patented technique allows for efficient extracting large amounts (upwards 10 million) of genetic material from small sample sizes without requiring complicated steps or equipment that are difficult to operate manually. It also has several benefits including faster processing time by allowing it to perform multiple processes on one device instead of three separate devices, reduced costs compared to current methods like liquid phase microextraction, and less harmful chemical exposure during sampling procedures.

Problems solved by technology

Technological Problem: Current methods used for extracting pure or enriched plasma (P)DNA involve complicated procedures such as sedimentations, centripetings, and others that can be time-consuming and costly. Additionally, current techniques may result in poor results due to factors like contamination during collection process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Configuration of lysate 1: first add deionized water to the volumetric flask, add guanidine hydrochloride with a concentration of 4mol / L, 3% Triton-100 (V / V), and 5% Tween-20 (V / V), 3% Chelex-100 (m / V), 6mmol / L disodium edetate, 40mmol / L trishydroxymethylaminomethane, dilute to the required volume with deionized water volume, adjust the pH value to 4.5 with hydrochloric acid;

[0040]2) Configuration of lysate 2: 20 mg / ml proteinase K, 50 mmol / L trimethylaminomethane, hydrochloric acid to adjust the pH of the solution to 8.0;

[0041] 3) Configuration of the binding solution: first add deionized water to the volumetric flask, add sodium chloride with a concentration of 1.5mol / L, 50% isopropanol (V / V), and dilute to the desired volume with deionized water. required volume;

[0042] 4) Configuration of washing liquid I: first add deionized water into the volumetric flask, add guanidine hydrochloride with a concentration of 3 mol / L, sodium chloride with a concentratio...

Embodiment 2

[0052] 1) Configuration of lysate 1: first add deionized water to the volumetric flask, add guanidine hydrochloride with a concentration of 6mol / L, 7% Triton-100 (V / V), and 3% Tween-20 (V / V), 6% Chelex-100 (m / V), 3mmol / L disodium edetate, 70mmol / L trishydroxymethylaminomethane, dilute to the required volume with deionized water volume, adjust the pH value to 4.5 with hydrochloric acid;

[0053] 2) Configuration of lysate 2: 20 mg / ml proteinase K, 50 mmol / L trimethylaminomethane, hydrochloric acid to adjust the pH of the solution to 8.0;

[0054] 3) Configuration of the binding solution: first add deionized water to the volumetric flask, add sodium chloride with a concentration of 2mol / L, 70% isopropanol (V / V), and dilute to the required volume with deionized water volume;

[0055] 4) Configuration of washing liquid I: first add deionized water into the volumetric flask, add guanidine hydrochloride with a concentration of 6 mol / L, sodium chloride at 2 mol / L, and 20% isopropan...

Embodiment 3

[0065] 1) Configuration of lysate 1: first add deionized water into the volumetric flask, add guanidine hydrochloride with a concentration of 8mol / L, 9% Triton-100 (V / V), and 10% Tween-20 (V / V), 10% Chelex-100 (m / V), 9mmol / L disodium edetate, 100mmol / L trishydroxymethylaminomethane, dilute to the required volume with deionized water volume, adjust the pH value to 4.5 with hydrochloric acid;

[0066] 2) Configuration of lysate 2: 20 mg / ml proteinase K, 50 mmol / L trimethylaminomethane, hydrochloric acid to adjust the pH of the solution to 8.0;

[0067] 3) Configuration of the binding solution: first add deionized water to the volumetric flask, add sodium chloride with a concentration of 3mol / L, 90% isopropanol (V / V), and dilute to the required volume with deionized water volume;

[0068] 4) Configuration of washing liquid I: first add deionized water into the volumetric flask, add guanidine hydrochloride with a concentration of 8 mol / L, sodium chloride with 3 mol / L, and 30% is...

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PUM

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Abstract

The invention discloses a whole blood genomic DNA high-flux plate type extracting kit and an extracting method, and relates to the technical field of biology. The whole blood genomic DNA high-flux plate type extracting kit comprises a lysate 1, a lysate 2, a combination liquid, and magnetic beads, wherein the lysate 1 comprises the following components: 2 to 8 mol/L guanidine hydrochloride, 1 to 10 percent of triton-100 (V/V), 1 to 10 percent of Tween-20 (V/V), 1 to 10 percent of chelex-100 (m/V), 2 to 10 mmol/L ethylene diamine tetraacetic acid and 20 to 100 mmol/L tromethamine, wherein the pH is regulated to be 4.5 by hydrochloric acid, and the solvent is deionized water; the lysate 2 comprises the following components: 20 mg/ml protease K and 50 mmol/L trimethyl aminomethane, wherein the pH is regulated to be 8.0 by the hydrochloric acid; the combination liquid comprises the following components: 1 to 3 mol/L sodium chloride and 40 to 90 percent of isopropyl alcohol (V/V), wherein the solvent is the deionized water. According to the whole blood genomic DNA high-flux plate type extracting kit and the extracting method, the purpose of extracting 96 samples simultaneously within 50 minutes can be achieved without centrifuging or matching an automatic instrument.

Description

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Claims

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Application Information

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Owner LUOYANG G N T BIOLOGICAL TECH CO LTD
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