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Application of bacillus mojavensis KJS-3 to decomposition of phenol

A technology of bacillus and purpose, applied in the field of wastewater treatment, can solve the problems of decreased microbial activity, decreased wastewater treatment efficiency, low microbial efficiency, etc., and achieves the effect of improving decomposition ability

Inactive Publication Date: 2017-05-17
武汉虹睿生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the efficiency of existing microorganisms in this metabolic process is very low, and various wastes contained in wastewater are difficult to decompose and metabolize quickly.
Moreover, if these intermediate substances are dissolved in wastewater, the activity of related microorganisms will be reduced, which will further reduce the efficiency of wastewater treatment, and because the toxic components of those chemicals are discharged into nature, it will also cause secondary environmental pollution.

Method used

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  • Application of bacillus mojavensis KJS-3 to decomposition of phenol
  • Application of bacillus mojavensis KJS-3 to decomposition of phenol
  • Application of bacillus mojavensis KJS-3 to decomposition of phenol

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Experimental program
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Effect test

Embodiment 1

[0015] The isolation of embodiment 1 bacterial strain

[0016] The viscous substances that mold cannot grow in the food waste were collected, moved to the laboratory, and cultivated in a shaker in tryptic soy agar medium (TSA) at 37°C for 16 hours. Here, the growing Colonies were inoculated in Trypticase Soy Broth Medium (TSB) for storage, and the grown strains were kept at 45°C and 20°C for culture, and then the strains grown out of the culture were selected and then kept .Store these selected strains at 4°C for 30 days, and then culture them again at 37°C in tryptic soy agar liquid medium (TSA). Two of the strains that had formed endospores (Endospore) in 7 days were selected, and the strains were named No. 3 and No. 4 strains respectively.

[0017] These strains were respectively entrusted to the Korea Microorganism Collection Center for genetic material analysis to obtain the Mojave Bacillus KJS-3 (Bacillus mojavensis KJS-3) and Mojave Bacillus KJS-4 (Bacillus mojavensis ...

Embodiment 2

[0018] Embodiment 2 has the confirmation of phenol decomposition ability

[0019] The above-mentioned two kinds of microorganisms, Bacillus mojavensis KJS-3 (Bacillus mojavensis KJS-3) and Bacillus mojavensis KJS-4 (Bacillus mojavensis KJS-4), were placed at a temperature of 40°C on tryptone soybean agar medium (TSA ) inoculation culture. This experiment was carried out using Bacillus mojavensis KJS-3 (Bacillus mojavensis KJS-3) cultured at 40°C.

[0020] 1. Inoculate the Bacillus Mojave KJS-3 strain in 10ml TSB medium, and culture at 40°C for more than 16 hours.

[0021] After culturing for 16 hours, take 5ml out of the culture solution, inoculate in 100ml TSB, and incubate at 40°C for 90 minutes, with the stirring speed kept at 100-200rpm.

[0022] 2. Take 500 μl (microliter) of the culture cultured in the above steps and centrifuge at 10,000 rpm for 1 minute to remove the supernatant, then inject 500 μl of normal saline (0.9% NaCl), mix completely and inoculate in minimal...

Embodiment 3

[0029] For the determination of the phenol content of the phenol-decomposing strain, the reaction experiment was carried out according to the following sequence, and the absorbance was measured at a wavelength of 650 nm.

[0030] 1) After the strain has been cultured for 8 hours, transfer the culture solution to the refrigerator for storage.

[0031] 2) Quantification of phenol

[0032] (1) Standard product preparation: 250ppm-phenol, 25ppm-phenol, 12.5ppm-phenol.

[0033] (2) Sample: 500ppm-phenol, containing a small amount of culture medium (cultivated at 40°C for 8 hours);

[0034] 250ppm-phenol contains a small amount of medium, and 50ppm-phenol contains a small amount of medium (cultivation at 40°C for 8 hours).

[0035] (3) Experimental process: 100ml of the standard substance and each sample were respectively taken, and 6ml of 8wt% K was added 3 Fe(CN) 6 , 6ml of 0.1M FeCl 3 (0.1M HCl as a solvent), after reacting for 5 minutes, the absorbance value (OD) was measur...

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Abstract

The invention relates to application of bacillus mojavensis KJS-3 to decomposition of phenol, and belongs to the field of wastewater treatment. Removal of phenolic compounds is a key issue in wastewater treatment. The invention mainly focuses on identification of a strain for efficiently degrading the phenol, namely the bacillus mojavensis KJS-3 (with the strain collection number of KCCM10961P). Experiments prove that the strain is a potential environment-friendly strain having the capability of degrading the phenol in wastewater.

Description

technical field [0001] The invention relates to the use of Mojave bacillus KJS-3 for decomposing phenol, and belongs to the field of wastewater treatment. Background technique [0002] Nowadays, various industrial wastewater contains not only organic substances but also refractory compounds that are difficult to decompose. Among these refractory compounds, not only are they toxic to the human body, but also become one of the main substances causing environmental pollution due to their difficulty in natural decomposition, resulting in the emergence of their environmental residues. Especially with the rapid development of the chemical industry, aromatic compounds used in various processes have a benzene ring structure, which is not only toxic to most of the human body, but also becomes one of the main causes of environmental pollution due to difficulty in decomposition in nature One, and its use is still increasing. Phenol has been identified as a major pollutant by the U.S....

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F101/34
CPCC02F3/34C02F2101/345
Inventor 姜在璿
Owner 武汉虹睿生物科技开发有限公司
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