Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for refining and purifying RB (Rebaudioside B)

A technology for refining products, stevia extracts, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve problems such as insufficient research, and achieve the effect of simple process and low cost

Inactive Publication Date: 2017-05-17
CHENGUANG BIOTECH GRP CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on steviol glycoside RB is still insufficient. In the patent publication number CN 10544091 A, a method for removing stevioside RB by anion exchange resin is reported. This patent reports how to remove RB in stevioside, and how to refine Purification of RB was not reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for refining and purifying RB (Rebaudioside B)
  • Method for refining and purifying RB (Rebaudioside B)
  • Method for refining and purifying RB (Rebaudioside B)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 100ml of gel-type cation exchange resin and put it into the chromatographic column, and pretreat the resin according to the following steps: use 2BV 4% hydrochloric acid to pass through the cation exchange resin, wash with water until the pH is 6.65; then use 2BV 4% sodium hydroxide aqueous solution to pass The cation exchange resin is washed with water until the pH is 8.52; finally, 2BV of 4% hydrochloric acid is passed through the cation exchange resin, and 5BV of water is used for washing. Take 70g rich in RB stevioside (stevia rebaudiana extract) (total glycoside sugar content is 69.92%, of which RB content is 17.14%, see figure 1 ) dissolved in water. Pass the high RB solution through the chromatographic column and collect the effluent, stand to crystallize at 4°C, and filter and separate after 72 hours to obtain RB stevioside (93.43% of the total glycoside content, of which RB content is 87.70%, see figure 2 ).

Embodiment 2

[0027] Take 100ml of macroporous cation exchange resin and put it into the chromatographic column, and pretreat the resin according to the following steps: use 2BV 4% hydrochloric acid to pass through the cation exchange resin, wash with water until the pH is 6.37; then use 2BV 4% sodium hydroxide aqueous solution to pass The cation exchange resin is washed with water until the pH is 8.74; finally, 4BV of 4% hydrochloric acid is passed through the cation exchange resin, and 8BV of water is used for washing. Take 100g rich in RB stevioside (stevia rebaudiana extract) (total glycosidic sugar content is 59.46%, wherein RB content is 24.58%, see image 3 ) dissolved in water. Pass the high RB solution through the chromatographic column and collect the effluent, stir at -4°C at 20r / s to precipitate crystals, filter and separate after 36h, and obtain RB stevioside (the total glycoside content is 96.25%, and the RB content is 90.66% ,See Figure 4 ).

Embodiment 3

[0029] Take 100g rich RB stevioside (stevia extract) (total glycoside sugar content is 74.91%, of which RB content is 57.59%, see Figure 5 ) dissolved in water. Add 30ml of 4% hydrochloric acid to the high RB solution, stir at 40r / s at 10°C to precipitate crystals, and filter and separate after 12h to obtain RB stevia (the total glycoside content is 90.39%, of which RB content is 85.85%, see Figure 6 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for refining and purifying RB (Rebaudioside B) and belongs to the technical field of refinement of natural extracts. The method comprises steps as follows: a solution containing RB is obtained by dissolving a stevia rebaudiana extract in water, the solution is supplemented with H<+> for crystallization, and a crystallized product, namely, a refined RB product, is obtained through solid separation. The method adopts a simple process and controllable conditions, and a stevioside product with the content of RB being 85% or higher can be obtained.

Description

technical field [0001] The invention belongs to the technical field of refining natural extracts, in particular to a method for refining and purifying rebaudioside B. Background technique [0002] Stevioside is a high-sweet, low-calorie, safe and non-toxic terpene glycoside extracted from the leaves of Stevia rebaudiana, an herb of Compositae, mainly including stevioside (Stevioside referred to as ST), rebaudioside A (Rebaudioside A is referred to as RA), Rebaudioside B (Rebaudioside B is referred to as RB), Rebaudioside C (RebaudiosideC is referred to as RC) and other glycoside ingredients. Various components in steviol glycosides have different taste, taste, and sweetness due to different molecular structures, and have different applications in food, medicine, and daily chemical industries. The preparation of high-purity RA, RB, and SB has become the focus of subdivided applications. The inevitable trend. Among them, RB, as a component of total steviol glycosides, has th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H15/256C07H1/06C07H1/08
CPCC07H15/256C07H1/06C07H1/08
Inventor 牛志平张亭田洪高伟王艳芳
Owner CHENGUANG BIOTECH GRP CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com