A Method for SSR Analysis of Tetraploid Alfalfa Using Multiplex PCR

An alfalfa and tetraploid technology, which is applied in the directions of biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, can solve problems such as genetic diversity analysis of tetraploid alfalfa, and achieve easy identification. , high accuracy, uniform distribution effect
CN106676176BActive Publication Date: 2020-09-22TIANJIN AGRICULTURE COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TIANJIN AGRICULTURE COLLEGE
Publication Date
2020-09-22

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Abstract

The invention relates to a method for performing SSR analysis on tetraploid alfalfa by utilizing multiple PCR. The method specifically comprises nine multiple PCR-SSR groups (24 pairs of SSR primers) and corresponding PCR systems and reaction programs. 24 SSR markers are uniformly distributed on chromosomes, the distance between the markers being not smaller than 10Mbp; marker genotypes are easy to identify, and the average genotype accuracy rate is higher than 95%. When the solution is adopted to perform genetic diversity analysis, group structure analysis and variety identification on the tetraploid alfalfa, the method has the advantages of short time, low cost and high accuracy, and the total efficiency can be improved by two to three times.
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Description

technical field

[0001] The invention relates to a method for SSR analysis of tetraploid alfalfa by multiplex PCR, and belongs to the field of biotechnology. Background technique

[0002] Alfalfa (Medicago sativa) is the most important forage crop in temperate regions of the world. The two main planted subspecies (M.sativa subssp.sativa and M.sativa subssp.×varia) are all autotetraploid, natural outcrossing , severe inbreeding depression. Genetic diversity is the basis for plant breeding and genetic improvement. Since most alfalfa varieties are synthetic varieties, they are bred by continuous random mating of selected excellent individual plants and their progeny for several generations. Therefore, when analyzing genetic diversity, generally 20 to 40 individual plants should be selected from one alfalfa variety. When many species are studied, this often results in a very large number of analysis units (genotypes). Due to the limitations of actual workload, funds, time and...

Claims

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