SNP (single nucleotide polymorphism) marker related to rapid growth of micropterus salmoide L. 'excellent bass No. 1' and application of SNP marker

A largemouth bass and marking technology, which is applied in the direction of microbial determination/inspection, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of cannibalism, parental quality of largemouth bass, and large growth disparity , to achieve the effects of fast growth, reduced blindness, and stable genetics

Active Publication Date: 2017-05-24
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is convenient, fast, and simple, but it is greatly affected by human factors. In addition, largemouth bass is a carnivorous fish with strong predatory nature. If the bait is insufficien

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Acquisition of SNP markers related to the rapid growth of largemouth bass "Youzhu No. 1"

[0024] 1. Acquisition of SNP markers

[0025] The new breeding species of largemouth bass "Youyu No. 1" is a largemouth bass obtained through 5 consecutive generations of selective breeding based on 4 breeding populations in China. Breeding species of perch. The growth rate of this new species is 17.8%-25.3% faster than that of common largemouth bass, and the deformity rate of high back and short tail is reduced from 5.2% to 1.1%. This variety was approved by the National Aquatic Original Species and Improved Breeds Approval Committee in 2010, and the approval number is GS01-004-2010. During the breeding process, we compared the production performance of the selective breeding group and the non-selective breeding group of largemouth bass. We used metal wire code marks to mark 800 breeding groups and non-selective breeding groups that were close to the time of breeding,...

Embodiment 2

[0036] Example 2 Application of the above-mentioned SNP sites in screening fast-growing largemouth bass parents

[0037] Utilize above-mentioned SNP locus to screen fast-growing largemouth bass parent, comprise the following steps:

[0038] 1) Cut fin ray samples from the parent to be tested and extract DNA;

[0039] 2) Using the extracted DNA as a template, use specific primers for primary PCR amplification to obtain primary PCR products,

[0040] 3) Use the Snapshot method to use the first-time PCR product as a template, use the extension primer to extend a base to terminate at the polymorphic site, and detect it on the sequencer. According to the color of the peak, the base type of the polypeptide site can be known to determine the target to be tested Whether the parents are homozygous.

[0041] The reaction system for the initial PCR amplification is:

[0042] dna 1μl 10×buffer 1.5μl 25mmol of MgCl 2

1.5μl dNTP 0.3μl 20P upstrea...

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) marker related to rapid growth of micropterus salmoide L. 'excellent bass No. 1' and application of the SNP marker. The SNP marker is respectively shown as SEQ ID NO.1, SEQ ID NO.2 or SEQ ID NO.3; the 658th base of SEQ ID NO.1 is C or A from 5' terminal, and the growth speed of CC individuals is obviously higher than that of AA individuals; the 327th base of SEQ ID NO.2 is A or G from 5' terminal, and the growth speed of AA individuals is obviously higher than that of GG individuals; and the 121th base of SEQ ID NO.3 is A or G from 5' terminal, and the growth speed of the AA individuals is obviously higher than that of the GG individuals. According to the application of the three SNP markers discovered in the invention, the parental line selection blindness of the micropterus salmoide L. 'excellent bass No. 1' can be greatly reduced, and micropterus salmoide L. individuals with high growth speed and genetic stability can be rapidly obtained.

Description

technical field [0001] The invention belongs to the field of molecular biology technology, and in particular relates to a SNP marker related to the rapid growth of largemouth bass "Youzhu No. 1" and an application thereof. Background technique [0002] Largemouth Bass ( Micropterus salmoides L.), commonly known as California perch, is native to North America. It has the characteristics of fast growth, low temperature resistance, delicious meat and easy fishing. It is one of the important freshwater cultured fish. Introduced from Taiwan to Guangdong Province in 1983, it is now cultivated in most parts of the country. However, at present, the largemouth bass cultured in my country is domesticated from wild species, lacks directional selection, and often selects small individuals and early sexually mature individuals as parents when the parents are kept, resulting in the germplasm of largemouth bass in my country. The decline is mainly manifested in the decrease of growth rat...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/158
Inventor 李胜杰樊佳佳姜鹏王俊杰胡婕
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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