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A kind of method for rapid propagation of fan fern tissue culture with young sporangia as explants

A technology of sporangia and explants, which is applied in the field of rapid propagation of fern tissue culture, which can solve the problems of low reproductive efficiency, long reproductive cycle, and difficulty in obtaining sterile spore explants, so as to shorten the reproductive time and improve the reproductive efficiency Effect

Active Publication Date: 2018-12-25
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of the low natural fecundity of the fan fern, and the existing tissue culture of the fan fern requires at least 5-6 explants from mature spores to germinate and form prothallium → prothallium proliferation → prothallus induction to obtain sterile sporophyte seedlings. months, and the induction rate of sterile sporophytic seedlings is only about 30%, the reproductive cycle is long, the reproductive efficiency is low, and the technical problem that suitable sterile sporophytic explants are difficult to obtain, the invention provides a kind of sporangia with young sporangia as the external Rapid Propagation Method of Pteridophyllum Tissue Culture by Implants

Method used

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  • A kind of method for rapid propagation of fan fern tissue culture with young sporangia as explants
  • A kind of method for rapid propagation of fan fern tissue culture with young sporangia as explants
  • A kind of method for rapid propagation of fan fern tissue culture with young sporangia as explants

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Effect test

Embodiment 1

[0030] Embodiment 1 The inventive method

[0031] (1) Obtaining the explants of aseptic young sporangia of P.

[0032] Select fern leaves that are growing well and have young sporangia, and use a soft brush dipped in 1% volume fraction of detergent water to clean the surface of the young sporangia of the fern, and remove the young sporangia of the fern Small scales and sundries on the surface, until the yellow-green fern sporangia can be seen, then carefully peel off the fern sporangia group from the blade with a scalpel blade, place it in a beaker, and rinse with running water for 1 hour. Then, under aseptic conditions, the aseptic young sporangia of Pteris fern were sterilized with 5% volume fraction of sodium hypochlorite solution for 12 minutes, rinsed with sterile water for 5 times, each time for 5 minutes, and then the aseptic young sporangia of Pteris fern were explanted. body.

[0033] The fern sporangia group is a fern sporangia group that grows together in groups. ...

Embodiment 2

[0048] Embodiment 2 and embodiment 3 are the method of the present invention

[0049] Embodiment 2 and embodiment 3 are different except that the measures listed in table 1 are different, and all the other measures are the same as embodiment 1, and will not be repeated.

[0050] The difference between the steps of Table 1 Embodiment 2-Embodiment 3 and Embodiment 1

[0051]

[0052]

[0053] The propagation effect of embodiment 1-3 is shown in table 2.

[0054] The reproductive effect of table 2 embodiment 1-embodiment 3

[0055]

[0056] In each of the above embodiments, a 2 mm green spheroid can be differentiated once to obtain 15 to 20 plantlets of the plantlets, and the reproduction rate of the plantlets of the plantlets is particularly high.

[0057] Above-mentioned embodiment 1 to embodiment 3 show: the present invention directly uses the aseptic young sporangia of fan fern as explants, and it only takes 42 days to 50 days to induce the green green spheroids of...

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Abstract

The invention discloses a rapid tissue culture propagation method of neocheiropteris palmatopedata by taking young sporophyte sorus as an explant. The method comprises the following steps: obtaining a sterile young sporophyte sorus explant of the neocheiropteris palmatopedata, inducing a green globular body, proliferating the green globular body, differentiating the green globular body, culturing a tissue culture seedling and rooting. The method directly takes the sterile young sporophyte sorus explant of the neocheiropteris palmatopedata as the explant and the green globular body can be induced after only 42 days to 50 days, so that an induction period of the green globular body is shortened and the propagation efficiency is high; the induction rate of the green globular body reaches 96 percent to 100 percent and the multiplication multiple reaches 6.21 to 7.47 times; the differentiating rate of the green globular body reaches 100 percent and the rooting rate reaches 100 percent; the propagation period is short; the rapid tissue culture propagation method has important meanings on expansion of the population quantity of rare and endangered plants, namely the neocheiropteris palmatopedata, and sustainable development and utilization of neocheiropteris palmatopedata wild resouces.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for rapid propagation of fern by tissue culture using young sporangia as explants. Background technique [0002] Fan fern [Neocheiropteris palmatopedata (Barker) Christ], belonging to Polypodiaceae (Polypodiaceae), herbaceous plant. The rhizomes are thick and horizontal, densely covered with scales; the leaves are fan-shaped, bird-like palm-shaped splits, peculiar in shape, and have high ornamental value. It is a special fern in China. It is produced in Yunnan, Sichuan, and Guizhou. It grows in dense forests or cliff forests at an altitude of 1500-2700 meters. Fan fern not only has ornamental value, but also has important research value in the development of fern phylogeny. However, due to the destruction of habitat, the number of wild distribution populations has decreased sharply. It is a national second-level key protected wild plant. [0003] Field ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 余蓉培杨春梅张颢阮继伟吴丽芳汪国鲜单芹丽
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI