The incubation method of Taiwanese loach fertilized eggs without changing the water throughout the process
A technology of fertilized eggs and loach, applied in the direction of climate change adaptation, application, fish farming, etc., can solve the problems of many deformed seedlings and weak seedlings, and achieve the effects of high seedling emergence rate, robust seedlings and reduced water consumption.
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Embodiment 1
[0021] 1. Obtaining fertilized eggs of Taiwan loach
[0022] Select the mature species of loach that has been bred for more than one year. When oxytocin is induced, the species of loach are weighed first, and the parents are paired according to the ratio of 20:1 (♀:♂) to calculate and prepare the oxytocin hormone. Oxytocin is dissolved in normal saline, and the hormone compatibility scheme is: DOM15 mg per kg of female loach + LRH-A 2 40 micrograms. Female loach was injected with 0.5mL oxytocic drug once in the back muscle, while male loach was not injected. The water temperature is required to be 23°C, and the effect time is reached after 21 hours. Immediately dry the body surface of the loach with a soft towel, squeeze the loach's abdomen with hands, and squeeze the eggs from the cloaca into a dry basin. At the same time, the male loach was dissected, the testis was taken out, the sperm preservation solution was added, and it was ground in a stainless steel filter. Add ...
Embodiment 2
[0033] 1. Obtaining fertilized eggs of Taiwan loach
[0034] Select the mature species of loach that has been bred for more than one year. When oxytocin is induced, the species of loach are weighed first, and the parents are paired according to the ratio of 20:1 (♀:♂) to calculate and prepare the oxytocin hormone. Oxytocin is dissolved in normal saline, and the hormone compatibility scheme is: DOM15 mg per kg of female loach + LRH-A 2 35 micrograms. The female loach was injected with 0.5-1mL oxytocic drug once in the back muscle, and the male loach was not injected. The water temperature is 26°C, and the effect time is reached after 14 hours. Immediately dry the body surface of the loach with a soft towel, squeeze the loach's abdomen with your hands, and squeeze the eggs from the cloaca into a dry basin. At the same time, the male loach was dissected, the testis was taken out, the sperm preservation solution was added, and it was ground in a stainless steel filter. Add th...
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