Spica prunellae extract, and preparation method and application thereof

A technology of Prunella vulgaris and its extract, applied in the field of Prunella vulgaris extract and preparation, can solve the problem that the anti-senile dementia activity of Prunella vulgaris has not been reported, and achieves reduction of escape latency and number of crossing platforms, easy operation, and treatment of senile dementia. Effect

Inactive Publication Date: 2017-05-31
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current studies focus on the antihypertensive effect of Prunella vulgaris, but there is no report on the anti-senile dementia activity of Prunella vulgaris

Method used

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  • Spica prunellae extract, and preparation method and application thereof
  • Spica prunellae extract, and preparation method and application thereof
  • Spica prunellae extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The extraction method of Prunella vulgaris extract includes the following steps:

[0034] (1) Crush the Prunella vulgaris herbal materials, use a 70% volume concentration of ethanol aqueous solution as a solvent, and use a solvent reflux extraction method at a solid-liquid mass ratio of 1:6 to extract, extract 3 times, and filter to obtain the filtrate;

[0035] (2) Concentrate the filtrate to 65°C with a relative density of 1.08 to obtain concentrated liquid a;

[0036] (3) Add 3 times the volume of distilled water to concentrate a to obtain a suspension, then add 2 times the volume of the suspension with ethyl acetate, shake, stand still for layering, the supernatant is the ethyl acetate layer, and the lower liquid is equal volume Repeated extraction of the ethyl acetate from the mixture 3 times, combined the ethyl acetate layers, concentrated under reduced pressure to obtain Prunella vulgaris concentrate b; dried to obtain Prunella vulgaris extract.

Embodiment 2

[0038] The extraction method of Prunella vulgaris extract includes the following steps:

[0039] (1) Crush the Prunella vulgaris herbal material, use a methanol aqueous solution with a volume concentration of 70% as a solvent, and use an ultrasonic extraction method at a solid-liquid mass ratio of 1:4, extract 3 times, and filter to obtain the filtrate;

[0040] (2) Concentrate the filtrate to a relative density of 1.01 at 60°C to obtain concentrated liquid a;

[0041] (3) Add 2 times the volume of distilled water to concentrate a to obtain a suspension, then add 3 times the volume of the suspension with ethyl acetate, shake, stand still for layering, the supernatant is the ethyl acetate layer, and the lower liquid is equal volume Repeated extraction of the ethyl acetate of the mixture twice, combined the ethyl acetate layers, concentrated under reduced pressure to obtain Prunella vulgaris concentrate b; dried to obtain Prunella vulgaris extract.

Embodiment 3

[0043] The extraction method of Prunella vulgaris extract includes the following steps:

[0044] (1) Crush the Prunella vulgaris herbal material, use a 95% ethanol aqueous solution with a volume concentration of 95% as a solvent, and use a solid-liquid mass ratio of 1:8 to extract by percolation method, extract once, and filter to obtain the filtrate;

[0045] (2) Concentrate the filtrate to 65°C with a relative density of 1.10 to obtain concentrated liquid a;

[0046] (3) Add 5 times the volume of distilled water to the concentrated solution a to obtain a suspension, then add 1 times the volume of the suspension with ethyl acetate, shake, stand still for layering, the supernatant is the ethyl acetate layer, and the lower layer is the same volume Repeated extraction of the ethyl acetate of 5 times, combined the ethyl acetate layers, concentrated under reduced pressure to obtain Prunella vulgaris concentrate b; dried to obtain Prunella vulgaris extract.

[0047] The room temperature ex...

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Abstract

The invention discloses a spica prunellae extract, and a preparation method and application thereof. The weight percentage of caffeic acid in the spica prunellae extract is greater than or equal to 5 percent; the weight percent of rosmarinic acid is greater than or equal to 15 percent. The preparation method of the spica prunellae extract provided by the invention is simple, convenient and practical; the operation is easy; the spica prunellae extract is more suitable for industrial production; in addition, the extract has an improving effect on the study and memory of fast-aging model rats due to D-galactose; the escape latency period and the platform passing times of the fast-aging model rats in the water maze tests can be reduced; immunohistochemical results show that the extract can reduce the hippocampal CA1 area Caspase-3 and cerebral cortex GFAP expression of the fast-aging model rats. HE and Nissl dyeing results show that the extract can relieve the neuron injury due to the D-galactose. The condition that the spica prunellae extract has the effect of resisting Alzheimer's diseases is discovered; the spica prunellae extract is applied to the preparation of medicines for resisting the Alzheimer's diseases and for treating senile dementia.

Description

Technical field [0001] The invention belongs to the field of medicine preparation, and specifically relates to a Prunella vulgaris extract, a preparation method and an application. Background technique [0002] Alzheimer’s disease is a degenerative disease of the central nervous system and is the most common type of dementia. For patients with Alzheimer's disease, cognitive decline is the main sign of the disease. The pathological features of Alzheimer's disease mainly include amyloid plaques (formed by β-amyloid), neurofibrillary tangles, and neuron loss. According to statistics from the International Alzheimer’s Association, as of 2015, there are an estimated 47 million people with dementia in the world, of which Alzheimer’s disease accounts for about 60%-70%, and it is estimated that this number will exceed 100 million by 2050 . A set of statistics released by the United States in 2014 showed that the cost of care for patients with Alzheimer's disease has reached US$600 bil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/536A61P25/28
Inventor 高文远张静泽曲卓
Owner TIANJIN UNIV
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