Method for rapidly producing pyrethrin
A pyrethrin and fast technology, applied in the field of pesticides, can solve the problems of serious variety differentiation, unable to meet production needs, and content reduction, and achieve the effects of fast growth speed, fast cultivation speed, and environmental protection.
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[0021] The present invention will be further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited to following embodiment:
[0022] A method for rapidly producing pyrethrins, comprising the following steps:
[0023] (1) Select a clone individual as the mother plant, and take its flower buds as explants. When the flower buds grow to a length of 0.4-0.8 cm, that is, when the anthers are in the middle and late stages of mononuclei, soak the flower buds in 75% ethanol aqueous solution for 30 s, take it out and put it into 0.1 wt% mercuric chloride aqueous solution for 15 minutes, and then rinse it with sterile water 4 times;
[0024] (2) Under sterile conditions, extract 1g of anthers from flower buds and inoculate them in induction medium to induce callus. The induction medium is: MS +2,4-D 1.0 mg / L +BA 1.0 mg / L+ IAA 0.5 mg / L+NAA0.25 mg / L+sucrose 50 g / L+agar 7 g / L, conventionally autoclaved, pH value 5.4~5.6, cultu...
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