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A method for rapid production of pyrethrins

A pyrethrin, fast technology, applied in the field of pesticides, can solve the problems of serious variety differentiation, unable to meet production needs, content reduction, etc., and achieve the effects of fast growth speed, fast cultivation speed, and environmental protection

Active Publication Date: 2019-08-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pyrethrum is generally propagated mainly by seeds and cuttings, but the current varieties are severely differentiated and the content is reduced. At the same time, if the plant is infected by root-knot nematodes and viruses, it will become a poisonous plant and cannot be used. Based on the above reasons, currently relying on dried pyrethrum The method of extracting and processing the obtained pyrethrins can no longer meet the production needs

Method used

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited to following embodiment:

[0022] A method for rapidly producing pyrethrins, comprising the following steps:

[0023] (1) Select a clone individual as the mother plant, and take its flower buds as explants. When the flower buds grow to a length of 0.4-0.8 cm, that is, when the anthers are in the middle and late stages of mononuclei, soak the flower buds in 75% ethanol aqueous solution for 30 s, take it out and put it into 0.1 wt% mercuric chloride aqueous solution for 15 minutes, and then rinse it with sterile water 4 times;

[0024] (2) Under sterile conditions, extract 1g of anthers from flower buds and inoculate them in induction medium to induce callus. The induction medium is: MS +2,4-D 1.0 mg / L +BA 1.0 mg / L+ IAA 0.5 mg / L+NAA0.25 mg / L + sucrose 50g / L + agar 7 g / L, conventionally autoclaved, pH value 5.4~5.6, cu...

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PUM

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Abstract

The invention discloses a method for rapidly producing pyrethrin. The method comprises culturing callus directly from pyrethrum anther, and extracting pyrethrin from the callus through vortex oscillation and ultrasonic extraction. The method has a fast cultivation rate and short extraction time and can quickly and efficiently produce pyrethrin.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a method for rapidly producing pyrethrins. Background technique [0002] Pyrethrum (Py rethrum cinerariifolium Trev.) is native to Europe, and its research and utilization have a history of nearly one hundred years (Casida, 1973). At the beginning of this century, pyrethrum was widely cultivated all over the world. Introduced in provinces such as Yunnan and Yunnan, the species have recently degenerated, which directly affects the yield and quality of dried flowers. From the 1950s to the 1970s, the main producing countries of pyrethrum dried flowers in the world were once impacted by synthetic insecticides. Since the 1980s, with the enhancement of people's awareness of environmental protection, the application research of pyrethrum has been favored again. Pyrethrins obtained after extraction and processing of inflorescences (referred to as flower heads), because pyrethrins are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C67/48C07C67/56C07C69/747A01H4/00
CPCA01H4/005C07C67/48C07C67/56C07C69/747
Inventor 龙明华巫桂芬唐璇李朋欣张会敏
Owner GUANGXI UNIV
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