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Lactobacillus paracasei N1115 culture medium and its application

A technology of lactobacillus and paracheese, applied in the field of bioengineering, can solve problems such as difficulty in the production of bacterial powder, and achieve the effects of saving material and labor costs, low cost and simple ingredients

Active Publication Date: 2017-05-31
正阳君乐宝乳业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, my country's probiotics mainly rely on imports, and the production of domestic probiotic powder is difficult due to the existence of technical barriers
One of the big problems is the high-density cultivation of probiotics

Method used

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  • Lactobacillus paracasei N1115 culture medium and its application
  • Lactobacillus paracasei N1115 culture medium and its application
  • Lactobacillus paracasei N1115 culture medium and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0031] Embodiment 1-5 discloses a kind of Lactobacillus paracasei N1115 culture medium, and the raw material content of each embodiment is as shown in table 1 below:

[0032] Table 1 Raw material content table

[0033]

[0034] The method for cultivating Lactobacillus paracasei N1115 in the substratum of application embodiment 1-5 is as follows:

[0035] A, preparation of bacterium suspension: after Lactobacillus paracasei N1115 is activated for two generations, streak is cultivated on the solid MRS medium plate, cultivated for 24h at 37°C, and then cultivated for 24h at 37°C in MRS medium to obtain a concentration of 10 6 cfu / mL bacterial suspension.

[0036] b. Fermentation: Inoculate the above bacterial suspension into the Lactobacillus paracasei N1115 culture medium of Example 1-5 with an inoculum amount of 1%, and culture at 37° C. for 24 hours.

[0037] Further, the process of step a activating two generations is as follows:

[0038] Lactobacillus paracasei N1115 w...

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Abstract

The invention discloses Lactobacillus paracasei N1115 culture medium and also discloses a high-density culture method of Lactobacillus paracasei N1115. The Lactobacillus paracasei N1115 culture medium and its culture method have the advantages of good composition simplicity, simple configuration and low cost, material and manpower costs are saved, the concentration of live bacteria in fermentation broth acquired by culturing Lactobacillus paracasei N1115 with the Lactobacillus paracasei N1115 culture medium is 9.6*1010 cfu / mL.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a culture medium, in particular to a culture medium of Lactobacillus paracasei N1115, and at the same time, the invention also relates to a method for cultivating Lactobacillus paracasei N1115 at high density. Background technique [0002] At present, my country's probiotics mainly rely on imports. Due to the existence of technical barriers, the production of domestic probiotics powder is very difficult. One of the big problems is the high-density cultivation of probiotics. Research on high-density culture includes research on medium composition and culture conditions. [0003] The main components of the medium include: carbon source, nitrogen source, water, inorganic salts and growth factors. Carbon and nitrogen sources provide microorganisms with energy and the carbon and nitrogen elements needed to form themselves; water provides a stable growth environment for microorganisms; in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/245
CPCC12N1/20
Inventor 王世杰魏立华朱宏冯丽莉荀一萍张栋郝红伟薛玉玲
Owner 正阳君乐宝乳业有限公司
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