Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of cardiomyocytes

A cardiomyocyte and cell technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc.

Active Publication Date: 2019-11-12
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although researchers have continuously made breakthroughs in the fields of tissue and organ reconstruction, stem cells, biomaterials, and biomanufacturing, there is still no good method to promote the differentiation of stem cells into cardiomyocytes, which has become one of the bottlenecks in myocardial tissue engineering.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of cardiomyocytes
  • Preparation method of cardiomyocytes
  • Preparation method of cardiomyocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Preparation of cardiomyocytes using rat bone marrow mesenchymal stem cells

[0050] 1. Preparation of containers coated with conductive nanomaterials

[0051] Weigh 1 mg of single-walled carbon nanotubes (CNT, Nanjing Xianfeng Nano Material Technology Co., Ltd.) and put them into 1.5 ml EP tubes, then add 1 ml of collagen solution to each EP tube (collagen solution is collagen (Sigma company) dissolved The liquid obtained in water, the concentration of collagen in the collagen solution is 1.5 mg / mL), to obtain liquid 1; the liquid 1 was dispersed in an ice bath ultrasonically (ultrasonic breaker (HD2070, Bandelin, Germany)) for 5 minutes, and the ultrasonic power was 35% , so that the CNTs are evenly distributed in the collagen solution; then the sonicated liquid is dropped onto a small glass slide (150μL / 100mm 2 ) or on a 6-well cell culture plate, baked in a vacuum oven at 60°C for 24 hours, a layer of CNT film with uniform thickness will be formed on the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of myocardial cells. The preparation method of the myocardial cells comprises the steps as follows: stem cells are treated by using inflammatory cytokines in a container coated with a conductive nano-material to obtain the myocardial cells, wherein the proportion of the stem cells to the inflammatory cytokines is (0.5-1)*10<5>:1ng; the inflammatory cytokines are IFN-gamma and TNF-alpha, and the mass ratio of the IFN-gamma to the TNF-alpha is 1:1; and the conductive nano-material is a carbon nanotube. An experiment proves that the quantity of the myocardial cells obtained by processing the stem cells through the IFN-gamma and the TNF-alpha combined with the conductive nano-material is much greater than that obtained by processing the stem cells by separately using the IFN-gamma and the TNF-alpha, separately using the conductive nano-material and not utilizing the IFN-gamma and the TNF-alpha combined with the conductive nano-material. The myocardial cells can be prepared by using the IFN-gamma and the TNF-alpha combined with the conductive nano-material at proper concentration.

Description

technical field [0001] The invention relates to a method for preparing cardiomyocytes in the field of biotechnology. Background technique [0002] Cardiovascular disease has become one of the major diseases that threaten human life and health, and its morbidity and mortality are increasing rapidly year by year, and it is getting younger and younger. Among them, myocardial infarction and heart failure are the main causes of death from cardiovascular diseases. At present, the treatment methods for myocardial infarction mainly include medical treatment, interventional therapy, coronary artery bypass surgery and heart transplantation. However, these therapeutic methods have obvious limitations, therefore, exploring new therapeutic strategies and methods is urgently needed in the field of ischemic heart disease. [0003] The rapid development of tissue engineering technology provides a new idea and treatment strategy for repairing necrotic myocardial tissue and improving cardia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0657C12N2501/24C12N2501/25C12N2506/1353C12N2506/1384C12N2533/20
Inventor 江小霞王常勇董晓惠杨燕美王玉涵黄小会曹雪敏周瑾
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA