Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

In-vitro binding of immature dendritic cells mediated by NY-ESO-1 via polymeric structure domain

A technology of NY-ESO-1 and dendritic cells, applied in the field of biomedicine, can solve problems affecting the natural history of tumors

Inactive Publication Date: 2017-05-31
宁波美丽人生医药生物科技发展有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, the reduction of spontaneous NY-ESO-1 antigen in melanoma suggests that immune responses against NY-ESO-1 may influence the natural history of tumors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In-vitro binding of immature dendritic cells mediated by NY-ESO-1 via polymeric structure domain
  • In-vitro binding of immature dendritic cells mediated by NY-ESO-1 via polymeric structure domain
  • In-vitro binding of immature dendritic cells mediated by NY-ESO-1 via polymeric structure domain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] NY-ESO-1 protein can form multimeric structures even in loading buffer in the presence of regular concentrations of β-mercaptoethanol.

[0066] Experiments have found that NY-ESO-1 proteins obtained from bacteria and mammalian cells can form multimeric structures even in loading buffers with conventional concentrations of β-mercaptoethanol ( figure 1 A, B).

Embodiment 2

[0068] The polymerization reaction of NY-ESO-1 is mediated by its intermolecular disulfide bonds.

[0069] Cysteine ​​in amino acid residues 75, 76, 78, 152 and 165 were replaced with serine. Three types of NY-ESO-1 with amino acid substitutions were: ESOcs1 with cysteine-serine substitutions at positions 75, 76, and 78, ESOcs2 with substitutions at positions 152 and 165, and ESOcs3 with mutations at all five positions. Purify these proteins and use Western blot to compare the oligomerization state between them ( figure 1 B). The results showed that the wild-type protein clearly showed the presence of dimers, tetramers and even multimers of 130 kDa. ESOcs1 and ESOcs2 showed mainly monomer and dimer structures, while the result of ESOcs3 showed mainly monomer. This experiment shows that the polymerization of NY-ESO-1 is due to the disulfide bonds between its molecules.

Embodiment 3

[0071] NY-ESO-1 binds to human and mouse immature dendritic cells in vitro and is related to its own polymeric structure.

[0072] Purify these proteins and use Western blot to compare the oligomerization state between them ( figure 1 B). The results showed that the wild-type protein clearly showed the presence of dimers, tetramers and even multimers of 130 kDa. ESOcs1 and ESOcs2 showed mainly monomer and dimer structures, while the result of ESOcs3 showed mainly monomer. This experiment shows that the polymerization of NY-ESO-1 is due to the disulfide bonds between its molecules. NY-ESO-1 present in cancer cells was also analyzed by Western blot for its protein aggregation in the loading buffer containing conventional concentrations of β-mercaptoethanol, and it was found that NY-ESO-1 mainly manifested as trimers and tetramers Dimeric and monomeric forms are rarely found (mutation 1C). Experiments show that NY-ESO-1 binds to human and mouse immature dendritic cells in vit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biological medicine, in particular to in-vitro binding of immature dendritic cells mediated by NY-ESO-1 via a polymeric structure domain. The inventor of the invention discovers that NY-ESO-1 protein can form a poly structure even in a loading buffer with the conventional concentration of beta-mercaptoethanol; a polymerization reaction of NY-ESO-1 is mediated by an intermolecular sulfide bond of NY-ESO-1; the in-vitro binding of NY-ESO-1 with the immature human and mouse dendritic cells is related to a polymerization structure of NY-ESO-1; TLR4 influences in-vitro binding of NY-ESO-1 with DCs in marrow; the binding of NY-ESO-1 with the immature human and mouse dendritic cells can also be achieved by a calprotectin effect; the oligomerization of a polymer reduces the binding capacity of TLR4 and NY-ESO-1; a polymer structure of NY-ESO-1 and TLR4 in a host participate in immunoglobulin antibody response; and Art v1 (wtArt) and CA9 gene immunogenicity is improved by NY-ESO-1 gene fusion expression. NY-ESO-1 is confirmed to mediate the in-vitro binding of the immature dendritic cells via the polymeric structure domain.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to NY-ESO-1 mediating the in vitro combination of self-immature dendritic cells through the polymerization domain. Background technique [0002] It has been postulated that innate immune system receptors may be involved in the initial autoimmune response to tumor-specific antigens (TAAs). Necrotic tumor cells release endogenous factors or damage-associated pattern molecules such as heat shock proteins, high mobility group box B1 (HMGB-1), and uric acid. These damage-patterning molecules alert the innate immune system via CD91, receptor for advanced glycation end products, TLR2 / TLR4, and interleukin-1 (IL-1) receptor, respectively. TAA itself is generally regarded as a bystander of the above-mentioned "danger signal", acting as an adjuvant in the initial stage of the anti-tumor immune response. According to this example, the spontaneous immune response is caused by new peptides produced ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0784C07K14/47
Inventor 田晓丽
Owner 宁波美丽人生医药生物科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com