A method of adding tomato juice to induce efficient accumulation of astaxanthin in Haematococcus pluvialis
A technology for Haematococcus pluvialis and tomato juice, applied in the biological field, can solve the problems of insecure yield, high equipment requirements, complicated operation, etc., and achieve the effects of increasing yield, low cost, and improving production efficiency
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Embodiment 1
[0014] Step 1. Preparation of algae liquid: use BBM medium (Hua Rucheng, 1986), culture at a temperature of 19°C, a light intensity of 2000lx, and a light-to-dark ratio of 12h / 12h, and culture until Haematococcus pluvialis cells under fluorescent lamps In the logarithmic growth phase, the cell concentration reached 2.85×10 5 Individual / mL, that is, the algae liquid obtained for induction culture;
[0015] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: first wash the tomatoes, sterilize the surface, peel them in a sterile environment, add equal quality sterile water to homogenate, remove the debris with four layers of sterile gauze, 10000r / min Centrifuge for 10min, take the supernatant to make tomato mother liquor for use. Add tomato juice mother liquid to the above-mentioned cultured algae liquid until the final content of tomato juice is 0.030L / L; The light-dark ratio was 24h / 0h and the compound stress condition of nutrient starvation was cultivated fo...
Embodiment 2
[0017] Step 1. Preparation of algae liquid: use BBM medium (Hua Rucheng, 1986), culture at a temperature of 21°C, a light intensity of 1800lx, and a light-to-dark ratio of 12h / 12h, and culture until Haematococcus pluvialis cells under fluorescent lamp In the logarithmic growth phase, the cell concentration reached 2.85×10 5 Individual / mL, that is, the algae liquid obtained for induction culture;
[0018] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: first wash the tomatoes, sterilize the surface, peel them in a sterile environment, add equal quality sterile water to homogenate, remove the debris with four layers of sterile gauze, 10000r / min Centrifuge for 10min, take the supernatant to make tomato mother liquor for use. Add tomato juice mother liquid to the above-mentioned cultured algae liquid until the final content of tomato juice is 0.029L / L; The light-dark ratio was 24h / 0h and the compound stress condition of nutrient starvation was cultivated for...
Embodiment 3
[0020] Step 1. Preparation of algae liquid: use BBM medium (Hua Rucheng, 1986), culture under the conditions of temperature 22°C, light intensity 2000lx, light-dark ratio 12h / 12h, and culture until Haematococcus pluvialis cells under fluorescent lamp In the logarithmic growth phase, the cell concentration reached 2.85×10 5 Individual / mL, that is, the algae liquid obtained for induction culture;
[0021]Step 2. First wash the tomatoes, sterilize the surface, peel them in a sterile environment, add equal quality sterile water to homogenate, remove the debris with four layers of sterile gauze, centrifuge at 10000r / min for 10min, and take the supernatant to prepare Use tomato mother liquor. Add tomato juice mother liquid to the above-mentioned cultured algae liquid until the final content of tomato juice is 0.032L / L; The culture was carried out for 18 days under the combined stress conditions of continuous fluorescent light and nutrient starvation (no nutrient salt was added dur...
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