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Fluorescence real-time quantitative PRC detection kit for serum miR-630 and application of miR-630

A detection kit, mir-630 technology, applied in the field of bioengineering, can solve problems such as limiting clinical application, and achieve the effect of simple ingredients

Inactive Publication Date: 2017-05-31
SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Application Information

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Problems solved by technology

[0005] Considering the indications and side effects of radiotherapy, this approach therefore limits its clinical application in post-traumatic HO prophylaxis

Method used

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  • Fluorescence real-time quantitative PRC detection kit for serum miR-630 and application of miR-630
  • Fluorescence real-time quantitative PRC detection kit for serum miR-630 and application of miR-630
  • Fluorescence real-time quantitative PRC detection kit for serum miR-630 and application of miR-630

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Embodiment Construction

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0029] The invention discloses the application of miR-630 and a fluorescent real-time quantitative PCR detection kit for detecting the content of miR-630 in serum.

[0030] Application of miR-630

[0031] The inventors of the present invention studied the expression level of miR-630 in the serum of patients with traumatic HO. At the same time, the patients who did not develop HO (heterotopic ossification) after elbow laxity (Ctrl1) and the patients with fracture healing were matched by age and sex. Patients (Ctrl2) were used as two co...

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Abstract

The invention provides a fluorescence real-time quantitative PRC detection kit for serum miR-630 and application of the miR-630. The miR-630 can be applied to the preparation of a kit used for obtaining early traumatic heterotopic ossification informatton; and the fluorescence real-time quantitative PRC detection kit for the serum miR-630 comprises a miR-630 specific primer and an internal reference U6 specific primer. The fluorescence real-time quantitative PRC detection kit for the serum miR-630 can rapidly and accurately detect the expression level of the miR-630 in target blood, thereby obtaining heterotopic ossification relevant information.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a fluorescent real-time quantitative PCR detection kit for serum miR-630 and the application of miR-630. Background technique [0002] microRNAs (miRNA, miR) is a small molecule, non-coding, single-stranded RNA molecule, which can bind to the untranslated region (3'UTR) of the corresponding target gene mRNA through specific base pairing, resulting in Degrade or inhibit its stability, so as to further regulate the expression of related target genes and participate in related cell proliferation, differentiation, apoptosis and other processes. [0003] Heterotopic ossification (HO), as a common complication after clinical trauma and surgery, has always been a major problem for clinical orthopedic surgeons, and its prevention and treatment have not been better resolved. When HO starts to form, its maturation trend is almost impossible to reverse. Once the formation of heteroto...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q1/6851C12Q2600/158C12Q2600/178
Inventor 孙仰白李青峰范存义黎逢峰蔡江瑜
Owner SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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