Method for measuring content of soluble sugar in lolium multiflorum

A technology of ryegrass multiflora and determination method, which is applied in the field of determination of soluble sugar content of ryegrass multiflora, can solve the problems of complex operation, time-consuming, screening out, etc., and achieve high detection accuracy, high detection efficiency and simple operation Effect

Inactive Publication Date: 2017-05-31
SICHUAN AGRI UNIV
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Problems solved by technology

At present, the traditional wet chemical method-anthrone-sulfuric acid colorimetric method is mainly used for the determination of forage WSC in China. A large number of ryegrass varieties (lines) or samples are screened out the varieties (lines) or samples with appropriate WSC content. Therefore, find out a method that can quickly measure the content of WSC in ryegrass multiflora. Breeding and forage quality control are of great significance

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  • Method for measuring content of soluble sugar in lolium multiflorum
  • Method for measuring content of soluble sugar in lolium multiflorum
  • Method for measuring content of soluble sugar in lolium multiflorum

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Embodiment

[0052] A. Establishment of a near-infrared prediction model for the soluble sugar content of Lolium multiflora; the near-infrared prediction model for the soluble sugar content of Lolium multiflora is established by the following method, specifically comprising the following steps:

[0053] a. Collecting ryegrass multiflora samples, the ryegrass multiflora samples are the ryegrass multiflora samples collected in 2015-2016, including 16 national examination varieties and 22 new lines, and the collection growth period includes tillering stage and jointing stage , booting stage, heading stage, flowering stage, fruiting stage, and maturity stage. The collection parts include stems, leaves, and whole plants. Nitrogen fertilizer cultivation method, a total of 403 samples of ryegrass multiflora were killed at 105°C for 20 minutes, dried at 65°C, pulverized into powder in a micro pulverizer and passed through a 40-mesh sieve to obtain ryegrass multiflora sample powder;

[0054] b. The...

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Abstract

The invention discloses a measuring method for quickly measuring content of soluble sugar in lolium multiflorum. The measuring method comprises the following steps of providing a predicting model for predicting actual content of soluble sugar in the lolium multiflorum based on an near-infrared spectrum technique; on the basis of known actual contents of more samples, collecting the near-infrared original spectrum of each sample, and establishing a quantitative analysis predicting model for predicting content of soluble sugar in the lolium multiflorum based on the near-infrared spectrum technique and a chemometrics method, and pretreating a to-be-measured lolium multiflorum sample, so as to obtain to-be-measured lolium multiflorum sample powder; collecting the near-infrared original spectrogram, and detecting after the near-infrared spectrum is collected, wherein the spectrum collection process time is short. The measuring method has the characteristics that the operation is simple, the detection is rapid, the detection efficiency is high, the detection accuracy is high, and the like; the measuring method is suitable for being popularized and applied to the field of nutrient quality evaluation of grass.

Description

technical field [0001] The invention relates to the field of pasture nutritional quality evaluation, in particular to a method for measuring the soluble sugar content of Lolium multiflora. Background technique [0002] Multifloral ryegrass (Lolium multiflorum Lam.) is an annual or perennial forage grass belonging to the Gramineae ryegrass family. It is suitable for growing in warm and humid regions. It is one of the excellent forage grasses with world cultivation significance. There are large areas of cultivation. In recent years, with the adjustment of the national agricultural industry structure, herbivorous animal husbandry has developed rapidly. Due to its high yield, strong growth in winter and spring, strong adaptability, and rich nutritional value, ryegrass multiflora has solved the problem of livestock feeding in winter and spring in southern my country. The problem of insufficient grass has played an extremely important role in the crop rotation of grain and grass i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563
CPCG01N21/359G01N21/3563
Inventor 张新全杨忠富马啸黄琳凯汪霞潘玲刘秋旭李华
Owner SICHUAN AGRI UNIV
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