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Nannochloropsis mutant strain and its application

A technology of Nannochloropsis and mutant strains, applied in the field of microbial engineering, to achieve the effects of improved resistance to diseases and insect pests, good breeding conditions, and good environmental adaptability

Active Publication Date: 2016-08-10
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Patent application CN102492626 discloses a Nannochloropsis sp. strain ENN1003A, which has the advantages of fast growth, high culture density, high oil content, and rich in long-chain unsaturated fatty acids such as EPA and protein , suitable for the production of biodiesel and the development of health products, medicines, and food, but there is still room for improvement in the performance of this algae strain

Method used

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  • Nannochloropsis mutant strain and its application
  • Nannochloropsis mutant strain and its application
  • Nannochloropsis mutant strain and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 Mutagenic screening, indoor culture and oil accumulation of mutant strain ENN11-2

[0022] Take fresh sterile algae liquid of the wild strain ENN1003A of Nannochloropsis sp. in logarithmic phase for EMS mutagenesis. The initial concentration of mutagenesis is 6.3×10 6 / ml, 1% (by volume) of EMS was added to the algae solution, and incubated at 37°C for 60 minutes in the dark with shaking. 10% (by mass) sterile NaS 2 O 3 The solution was terminated, washed with seawater culture medium for 3 times, cultivated in the dark for 33 hours, and cultured until the lethality was stable. The algae solution was plated at 300 / plate on f / 2 solid plate with DCMU concentration of 1.5uM, and individuals were picked. A total of 30 large single algae colonies were screened in the Erlenmeyer flask, and ENN11-2, which grew fast, was selected for evaluation.

[0023] Inoculate ENN11-2 green swimming cells in the logarithmic growth phase in the prepared seawater medium (see Table 1 for ...

Embodiment 2

[0038] Example 2 Outdoor cultivation and oil accumulation of mutant strain ENN11-2

[0039] Inoculate the algae in the logarithmic growth phase in a 50*50*5cm plate reactor, use seawater culture medium for cultivation, and place it in outdoor natural conditions for growth and cultivation. The natural conditions during the cultivation period are as follows: the cultivation period is 11 days, The temperature range from 9:00 to 17:00 is mainly between 25-37℃, and occasionally the highest temperature reaches 40℃. The range of illumination change from 9:00 to 17:00, the sun surface is mainly 200-700umol / m 2 / s, occasionally the light intensity reaches 800umol / m 2 / s. Access a certain percentage of CO during the day 2 (Purity above 95%), control the pH value between 7-9. Intermittent sampling during the cultivation process to determine the dry weight (see results image 3 ). After the cultivation, the algae liquid is collected, and the algae mud is obtained by centrifugation or natu...

Embodiment 3

[0042] Example 3 Mutant strain ENN11-2 has high CO content 2 Waste gas farming

[0043] The algae species in the logarithmic growth phase were inoculated in a 50*50*13cm plate reactor, cultured using seawater culture medium (no C source added), and placed in outdoor natural conditions for growth and culture. The natural conditions during the culture are as follows: During the experiment, the temperature reached 39°C and above for only 7 days. The rest of the time is mostly around 30℃, and the temperature drops to 20-30℃ in the later stage. During the experiment, 8 hours of double-sided visible light illumination reached 8000μmol / m 2 / s(6.25MJ / m 2 ) And above 14 days, 7000-8000μmol / m 2 / s has 4 days; 6600μmol / m 2 There are 10 days below / s and 3 days when it rains. The weather conditions were general, and days with better light accounted for 58% of the experimental days. Access a certain percentage of CO during the day 2 (Chemical plant waste gas, purity above 95%), control ...

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Abstract

The invention discloses a nannochloropsis sp. mutant strain ENN11-2 with a preservation number of CGMCC No.6009, and applications thereof. The nannochloropsis sp. mutant strain ENN11-2 can be used for preparing medicines or health products which are used for preventing and treating cardiovascular and cerebrovascular diseases; for producing or preparing food; for producing baits or animal feed; for producing biofuel and especially biodiesel; for treatment of sewage containing nitrogen and phosphor; for emission reduction of CO2; and for producing long-chain unsaturated fatty acid and especially EPA. Compared with wild strain, growth speed of the nannochloropsis sp. mutant strain ENN11-2 is increased 10%, EPA content is increased 10%, performance is relatively stable, environmental suitability is excellent, disease resistance and insect pest resistance are high, and outdoor validation of the nannochloropsis sp. mutant strain ENN11-2 is realized.

Description

Technical field [0001] The present invention relates to the field of microbial engineering. Specifically, the present invention relates to a Nannochloropsis sp. and its applications. Background technique [0002] The extensive use of fossil energy has greatly aggravated atmospheric pollution and climate change, as well as the increase in acid rain area. It is imperative to find new sustainable environmentally safe biological resources to produce biofuels to replace fossil fuels. Biodiesel (Biodiesel) is a kind of environmentally friendly biofuel prepared from unprocessed or used vegetable oil and animal fat through different chemical reactions. This biofuel can be used like diesel. Biodiesel is a proven fuel that can partially replace fossil diesel in terms of energy properties and is one of the most promising liquid biofuels. [0003] Microalgae is a kind of low-grade plants that grow in water with a wide variety and distribution. It is a cell factory driven by sunlight and abso...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12A61K36/05A61P9/00A23L33/00A23K10/30A01K97/04C12P7/64C02F3/32B01D53/84B01D53/62C12R1/89
CPCY02A50/20Y02P20/59Y02W10/37
Inventor 王媛媛李青邓平罗少敬吴洪
Owner ENN SCI & TECH DEV
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