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A glass capillary-based bovine serum albumin detection probe and its preparation method and application

A technology of bovine serum albumin and glass capillary, applied in the field of immunoassay, can solve the problems of cumbersome detection steps, harsh detection conditions, and high toxicity of reagents, and achieve the effects of low detection cost, strong practicability, and wide application

Inactive Publication Date: 2019-10-08
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current detection methods all require expensive instruments, and the detection steps are cumbersome, time-consuming, and the detection conditions are harsh, and the reagents used in the detection are highly toxic

Method used

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  • A glass capillary-based bovine serum albumin detection probe and its preparation method and application
  • A glass capillary-based bovine serum albumin detection probe and its preparation method and application
  • A glass capillary-based bovine serum albumin detection probe and its preparation method and application

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preparation example Construction

[0028] This embodiment provides a method for preparing a glass capillary-based bovine serum albumin detection probe, which includes:

[0029] (1) Activation step: mix GC with ammonia solution, soak at 65-90°C for 7-15min, then take out the glass capillary and mix with hydrogen peroxide solution, and soak at 65-90°C for 7-15min , and then wash the glass capillary with an organic solvent to obtain OH-GC;

[0030] The surface of ordinary GC is covered with organic matter and metal ions. If these components are not removed, the hydroxyl groups on the surface of GC will be in a passivated state, making it difficult to carry out subsequent reactions. Therefore, using ammonia water and hydrogen peroxide to soak the GC can make the hydroxyl groups on the surface of the GC in an activated state, which is conducive to the smooth progress of the subsequent modification steps.

[0031] After many tests and explorations, the inventor concluded that the above soaking conditions are: soakin...

Embodiment 1

[0056] This embodiment provides a bovine serum albumin detection probe based on a glass capillary. The synthetic route of this bovine serum albumin detection probe is as follows: figure 1 Shown, its preparation method is as follows:

[0057] (1) Activation step: mix GC with ammonia solution, soak at 65°C for 15min, then take out GC and mix with hydrogen peroxide solution, soak at 65°C for 15min, then wash GC with acetone to get OH-GC;

[0058] (2) Modification step: prepare 3-aminopropyltriethoxysilane solution with toluene as solvent, then mix 3-aminopropyltriethoxysilane solution with OH-GC, and stir at 30°C for 18h, The GC was then washed with THF to give NH 2 -GC;

[0059] (3) Bonding step: mix BSA with EDC and NHS, stir at 20°C for 1 h; then mix with NH 2 -GC were mixed, stirred at 30°C for 4h, and washed to obtain BSA-GC.

Embodiment 2

[0061] This embodiment provides a bovine serum albumin detection probe based on a glass capillary, and the preparation method of this bovine serum albumin detection probe is:

[0062] (1) Activation step: mix GC with ammonia solution, soak at 75°C for 10 minutes, then take out GC and mix with hydrogen peroxide solution, soak at 75°C for 10 minutes, then wash with acetone and tetrahydrofuran in sequence GC, get OH-GC;

[0063] (2) Modification step: prepare 3-aminopropyltriethoxysilane solution with toluene as solvent, then mix 3-aminopropyltriethoxysilane solution with OH-GC, and stir at 25°C for 20h, The GC is then washed sequentially with acetone, tetrahydrofuran and toluene to give NH 2 -GC;

[0064] (3) Bonding step: mix BSA with EDC and NHS, stir at 25°C for 2h; then mix with NH 2 -GC were mixed, stirred at 25° C. for 5 h, and washed to obtain BSA-GC.

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Abstract

The invention provides a glass capillary-based bovine serum albumin detection probe and its preparation method and application. In the preparation method of the bovine serum albumin detection probe, first use cheap ammonia solution and hydrogen peroxide solution to make The hydroxyl group on the surface of the glass capillary (GC) is in an activated state, and then the activated GC surface is modified with an amino group using 3-aminopropyltriethoxysilane to obtain NH 2 ‑GC; then bond BSA to NH 2 ‑GC, get BSA‑GC. The bovine serum albumin detection probe has simple preparation method, mild reaction conditions, easy realization and is suitable for large-scale industrial production. When the bovine serum albumin detection probe detects BSA, it utilizes the specific binding reaction between the antigen and the antibody, and at the same time uses HRP to label the antibody to achieve specific visual detection with high sensitivity.

Description

technical field [0001] The invention relates to the field of immune analysis, in particular to a bovine serum albumin detection probe based on a glass capillary and a preparation method and application thereof. Background technique [0002] In the process of culturing cells to produce vaccines, bovine serum is the main component of the medium. Since bovine serum albumin is often contained in bovine serum, it is very important to detect residual bovine serum albumin (BSA) in vaccines, which is directly related to the safety of biological products. However, the current detection methods all require expensive instruments, and at the same time, the detection steps are cumbersome, time-consuming, and the detection conditions are harsh, and the reagents used in the detection are highly toxic. Contents of the invention [0003] The first object of the present invention is to provide a bovine serum albumin detection probe based on a glass capillary, which has a stable structure a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 青琳森王乾龙丁立生梁健饶亮明李兴德蒋立银帅克
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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